Configuration

AlphaProteo, pooled over tested design systems

AlphaProteo binder design as configured for alphaproteo, pooled over tested design systems: All designs tested for a target, pooled over the v1, v1-improved-filter and v2 systems as Table 1 reports them.

43 evaluations · 43 results

Overview

An explanatory profile has not yet been reviewed for this record.

Consult the linked sources for architecture or protocol details. Missing evidence is not evidence of a missing capability.

Evaluations and results

43 evaluations · 43 results. Different protocols are not a single leaderboard.

Sorted by Dissociation constant (best per-target KD by HTRF among expressed hits, non-optimised designs) (lower is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Protocol and dataset	Dissociation constant (best per-target KD by HTRF among expressed hits, non-optimised designs)	Hit rate (yeast-display binding signal above 0.2, over designs tested; pooled over the AlphaProteo design systems tested for this target)	Hit rate (yeast-display binding signal above 1.3, over designs tested; pooled over the AlphaProteo design systems tested for this target)	Count (Observed HTRF binding)	Count (Successfully subcloned and expressed)	Count (Tested by HTRF)	Count (Yeast surface display hits selected for E. coli expression)
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target IL-7RA	0.082 (best in column)	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target PD-L1	0.18	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target VEGF-A	0.48	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target TrkA	0.96	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target IL-17A	8.4	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target BHRF1	8.5	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo best per-target binder affinity by HTRF, AlphaProteo binder design target SC2RBD	26	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target BHRF1	Not reported	Not reported	Not reported	2	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-17A	Not reported	Not reported	Not reported	3	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-7RA	Not reported	Not reported	Not reported	11	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target PD-L1	Not reported	Not reported	Not reported	16	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target SC2RBD	Not reported	Not reported	Not reported	14	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target TrkA	Not reported	Not reported	Not reported	5	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target VEGF-A	Not reported	Not reported	Not reported	20	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target BHRF1	Not reported	Not reported	Not reported	Not reported	2	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-17A	Not reported	Not reported	Not reported	Not reported	9	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-7RA	Not reported	Not reported	Not reported	Not reported	11	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target PD-L1	Not reported	Not reported	Not reported	Not reported	20	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target SC2RBD	Not reported	Not reported	Not reported	Not reported	17	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target TrkA	Not reported	Not reported	Not reported	Not reported	5	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target VEGF-A	Not reported	Not reported	Not reported	Not reported	31	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target BHRF1	Not reported	Not reported	Not reported	Not reported	Not reported	2	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-17A	Not reported	Not reported	Not reported	Not reported	Not reported	7	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-7RA	Not reported	Not reported	Not reported	Not reported	Not reported	11	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target PD-L1	Not reported	Not reported	Not reported	Not reported	Not reported	18	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target SC2RBD	Not reported	Not reported	Not reported	Not reported	Not reported	17	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target TrkA	Not reported	Not reported	Not reported	Not reported	Not reported	5	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target VEGF-A	Not reported	Not reported	Not reported	Not reported	Not reported	31	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target BHRF1	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	2
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-17A	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	9
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target IL-7RA	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	11
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target PD-L1	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	21
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target SC2RBD	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	18
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target TrkA	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	10
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo expression and HTRF confirmation funnel for yeast-display hits, AlphaProteo binder design target VEGF-A	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	31
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target BHRF1	Not reported	88% (best in column)	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 1.3 (IL-17A), AlphaProteo binder design target IL-17A	Not reported	Not reported	14%	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target IL-7RA	Not reported	25%	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target PD-L1	Not reported	15%	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target SC2RBD	Not reported	12%	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target TNF-alpha	Not reported	0%	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target TrkA	Not reported	9%	Not reported	Not reported	Not reported	Not reported	Not reported
AlphaProteo, pooled over tested design systemsEvaluation	AlphaProteo yeast-display binding screen, hit at binding signal above 0.2, AlphaProteo binder design target VEGF-A	Not reported	33%	Not reported	Not reported	Not reported	Not reported	Not reported

All 43 result rows with coverage, uncertainty and sources

Filter evaluations

Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target BHRF1	2 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for BHRF1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'BHRF1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	3 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-7RA	11 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for IL-7RA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'IL-7RA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target PD-L1	16 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for PD-L1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'PD-L1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target SC2RBD	14 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for SC2RBD (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'SC2RBD'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target TrkA	5 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for TrkA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'TrkA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target VEGF-A	20 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for VEGF-A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'VEGF-A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target BHRF1	2 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for BHRF1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'BHRF1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	9 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-7RA	11 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for IL-7RA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target PD-L1	20 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for PD-L1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'PD-L1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target SC2RBD	17 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for SC2RBD (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'SC2RBD'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target TrkA	5 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for TrkA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'TrkA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target VEGF-A	31 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for VEGF-A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'VEGF-A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target BHRF1	2 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for BHRF1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'BHRF1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	7 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-7RA	11 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for IL-7RA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'IL-7RA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target PD-L1	18 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for PD-L1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'PD-L1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target SC2RBD	17 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for SC2RBD (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'SC2RBD'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target TrkA	5 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for TrkA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'TrkA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target VEGF-A	31 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for VEGF-A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'VEGF-A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target BHRF1	2 Count (Yeast surface display hits selected for E. coli expression) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo yeast surface display hits selected for e. coli expression for BHRF1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Yeast surface display hits selected for E. coli expression', column 'BHRF1'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	9 Count (Yeast surface display hits selected for E. coli expression) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo yeast surface display hits selected for e. coli expression for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Yeast surface display hits selected for E. coli expression', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-7RA	11 Count (Yeast surface display hits selected for E. coli expression) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo yeast surface display hits selected for e. coli expression for IL-7RA (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Yeast surface display hits selected for E. coli expression', column 'IL-7RA'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target PD-L1	21 Count (Yeast surface display hits selected for E. coli expression) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo yeast surface display hits selected for e. coli expression for PD-L1 (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Yeast surface display hits selected for E. coli expression', column 'PD-L1'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Use this configuration

How it works, versions and access

Strengths, limitations and unresolved questions

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

0 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance

No evidence rows match these filters. Choose another scope or clear the search.

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release history

De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Original source · arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)

Download this release (gzip)

Technical metadata and extraction receipts

Stable ID: uc-binder-20261022-config-alphaproteo-pooled

areas: proteins-complexes; protein-structure
contexts: research
method types: specialist
access reuse: Closed system; run by the authors. Not available to reuse.
foundation model eligible: false
method role: design
parameters: All designs tested for a target, pooled over the v1, v1-improved-filter and v2 systems as Table 1 reports them
reported name: AlphaProteo, pooled over tested design systems
review: artifact sha256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42; method: source-hash-verification; independent-cell-check; ai-assisted-source-review; method note: Re-downloaded with curl (HTTP 200, 12,333,400 bytes) and the SHA-256 recomputed and matched. Own text layer made with pdftotext -layout (poppler 26.08.0), reproducing the extraction pass's 2,811 lines. Table 1, Table S2, Table S3 and Sections S1.2.1, S2, S4.1 and S4.2 read cell by cell and compared with every record. Column assignment re-derived arithmetically rather than trusted.; note: Reviewed in docs/reviews/use-cases/binder-2026-10-22.md. Automated AI review of the source transcription only; it is not human scientific review and nothing here was reproduced experimentally.; retrieval url: https://arxiv.org/pdf/2409.08022v1; reviewed at: 2026-10-22T15:00:00Z; reviewer: claude; reviewer note: Claude review agent, independent of the extraction agent; no human review claimed
source locator: Table 1, rows 'AlphaProteo'; Section S2 'For simplicity, the results in Table 1 are pooled over all tested designs for each target.'
version: arXiv:2409.08022v1

Related records

Suggest a correction