Dataset

AlphaProteo binder design target IL-17A

Interleukin-17A homodimer as the design target for the AlphaProteo binder design campaign, with the input structure and hotspot residues given in Table S1.

Evaluation results

8 evaluations · 8 results. Different protocols are not a single leaderboard.

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Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: AlphaProteo v1	Protocol: AlphaProteo yeast-display binding screen, hit at binding signal above 1.3 (IL-17A) Dataset: AlphaProteo binder design target IL-17A	14.3% Hit rate (yeast-display binding signal above 1.3, over designs tested) percent · higher Uncertainty: Not reported by the source: No interval is printed. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo v1 on IL-17A (yeast-display hit rate, Table S2) uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3 Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S2, 'Success (%)' block, row 'AlphaProteo v1', column 'IL-17A'
Configuration: AlphaProteo v1	Protocol: AlphaProteo best per-target binder affinity by HTRF Dataset: AlphaProteo binder design target IL-17A	8.4 Dissociation constant (best KD by HTRF among the designs tested for this design system) nanomolar · lower Uncertainty: Not reported by the source: No interval is printed. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo v1 on IL-17A (best KD by HTRF, Table S2) uc-binder-20261022-protocol-alphaproteo-htrf-kd Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S2, 'Binding KD (nM)' block, row 'AlphaProteo v1', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	3 Count (Observed HTRF binding) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo observed htrf binding for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Observed HTRF binding', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	9 Count (Successfully subcloned and expressed) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo successfully subcloned and expressed for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	7 Count (Tested by HTRF) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo tested by htrf for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Tested by HTRF', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits Dataset: AlphaProteo binder design target IL-17A	9 Count (Yeast surface display hits selected for E. coli expression) count · unknown Uncertainty: Not applicable: An exact count. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo yeast surface display hits selected for e. coli expression for IL-17A (Table S3) uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Yeast surface display hits selected for E. coli expression', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo yeast-display binding screen, hit at binding signal above 1.3 (IL-17A) Dataset: AlphaProteo binder design target IL-17A	14% Hit rate (yeast-display binding signal above 1.3, over designs tested; pooled over the AlphaProteo design systems tested for this target) percent · higher Uncertainty: Not reported by the source: No interval or replicate count is printed. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo on IL-17A, pooled over tested design systems (yeast-display hit rate, Table 1) uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3 Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1, 'Experimental success rate (%)' block, row 'AlphaProteo', column 'IL-17A'
Configuration: AlphaProteo, pooled over tested design systems	Protocol: AlphaProteo best per-target binder affinity by HTRF Dataset: AlphaProteo binder design target IL-17A	8.4 Dissociation constant (best per-target KD by HTRF among expressed hits, non-optimised designs) nanomolar · lower Uncertainty: Not reported by the source: No interval is printed. Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Evidence concern: excluded from comparisons Table S2 prints the RFdiffusion yeast-display success rate for IL-7RA as 16.8% and Table 1 rounds it to 17, but Section S4.1 states it in the text as '16% versus 33%'. Section S4.1 also states TrkA as '0% (versus 6%)' where Table S2 prints 0.0 and 6.3. The table values are recorded; the text rounds down rather than to nearest.De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1; Table S2; Section S4.1 Methods, coverage and source AlphaProteo on IL-17A, pooled over tested design systems (best KD by HTRF, Table 1) uc-binder-20261022-protocol-alphaproteo-htrf-kd Aggregation: Not reported De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table 1, 'Binding KD (nM)' block, row 'AlphaProteo', column 'IL-17A'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Research readiness

0 of 4 readiness checks met. These checks assess whether the evidence supports a reproducible investigation; a source-checked score alone does not meet them.

Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

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Missing or unresolved evidence

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Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
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Score meaning and direction are confirmed: not yet verified
Metrics are recomputed from the saved predictions: not yet verified
Sample annotations are recorded: not yet verified
Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

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Missing or unresolved evidence

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Score meaning and direction are confirmed: not yet verified
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Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
Independent validation data exist: not yet verified
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Verified: Not verified

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No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Dataset and evaluation context

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Evidence

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Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

7 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Assay Yeast surface display with flow cytometry; HTRF for KD of expressed hits Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `attributes.assay` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Population Binder designs generated against IL-17A, 50 to 140 amino acids long Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `attributes.population` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Reported name IL-17A Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `attributes.reported_name` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Source location Table 1, column 'IL-17A'; Table S1 Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `attributes.source_locator` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Version arXiv:2409.08022v1 Table S1 Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `attributes.version` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Description Interleukin-17A homodimer as the design target for the AlphaProteo binder design campaign, with the input structure and hotspot residues given in Table S1. Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `description` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact
Name AlphaProteo binder design target IL-17A Context-only references	De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) Original source ↗ Table 1, column 'IL-17A'; Table S1 Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages) Retrieved: 2026-10-22T11:53:00Z	not individually reviewed No individual claim review recorded Source has a recorded evidence concern. Consult its source page before using the claim. Audit details Field: `name` Source artifact SHA-256: `953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42` Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22. Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release history

De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Original source · arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)

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Technical metadata and extraction receipts

Stable ID: uc-binder-20261022-data-alphaproteo-il17a

areas: proteins-complexes; protein-structure
contexts: research
assay: Yeast surface display with flow cytometry; HTRF for KD of expressed hits
missing metadata: accession: note: Table S1 gives PDB IDs and hotspot residue numbers; they were not transcribed in this pass.; reason: unextracted
population: Binder designs generated against IL-17A, 50 to 140 amino acids long
reported name: IL-17A
review: artifact sha256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42; method: source-hash-verification; independent-cell-check; ai-assisted-source-review; method note: Re-downloaded with curl (HTTP 200, 12,333,400 bytes) and the SHA-256 recomputed and matched. Own text layer made with pdftotext -layout (poppler 26.08.0), reproducing the extraction pass's 2,811 lines. Table 1, Table S2, Table S3 and Sections S1.2.1, S2, S4.1 and S4.2 read cell by cell and compared with every record. Column assignment re-derived arithmetically rather than trusted.; note: Reviewed in docs/reviews/use-cases/binder-2026-10-22.md. Automated AI review of the source transcription only; it is not human scientific review and nothing here was reproduced experimentally.; retrieval url: https://arxiv.org/pdf/2409.08022v1; reviewed at: 2026-10-22T15:00:00Z; reviewer: claude; reviewer note: Claude review agent, independent of the extraction agent; no human review claimed
source locator: Table 1, column 'IL-17A'; Table S1
version: arXiv:2409.08022v1 Table S1

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