rewirebio.iobenchmarks
Evaluation

AlphaProteo successfully subcloned and expressed for IL-7RA (Table S3)

Developer-reported count transcribed from the pinned source; not reproduced.

Evaluation results

1 evaluation · 1 result. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: AlphaProteo, pooled over tested design systemsProtocol: AlphaProteo expression and HTRF confirmation funnel for yeast-display hits
Dataset: AlphaProteo binder design target IL-7RA
11 Count (Successfully subcloned and expressed)
count · unknown

Uncertainty: Not applicable: An exact count.

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

AlphaProteo successfully subcloned and expressed for IL-7RA (Table S3)

uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel

Aggregation: Not reported

De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024) · Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Research readiness

0 of 4 readiness checks met. These checks assess whether the evidence supports a reproducible investigation; a source-checked score alone does not meet them.

Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

Exact outcomes, predictions, identifiers and evaluator are connected.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified
  • Sample annotations are recorded: not yet verified
  • Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • A pinned run recipe exists: not yet verified
  • Compute requirements are estimated: not yet verified

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Independent validation data exist: not yet verified
  • Overlap with training data is checked: not yet verified

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Evaluation procedure

AlphaProteo expression and HTRF confirmation funnel for yeast-display hits

Configuration
AlphaProteo, pooled over tested design systems
Protocol
AlphaProteo expression and HTRF confirmation funnel for yeast-display hits
Dataset
AlphaProteo binder design target IL-7RA
origin
Author-reported evaluation
configuration
arXiv:2409.08022v1 Table S3
adaptation
Not reported
aggregation
Count per target per stage
budget
Not reported
dataset version
IL-7RA, Table S1 input structure
inputs
Not reported
metric implementation
Count of designs surviving the stage
population
Yeast-display hits selected by the authors for follow-up
protocol id
uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel
split
Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split
Not reported
Adaptation
Not reported
Scoring implementation
Count of designs surviving the stage

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

18 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Comparison: adaptation
Not reported
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

missing or unspecified

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: aggregation
Count per target per stage
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: budget
Not reported
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

missing or unspecified

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: dataset version
IL-7RA, Table S1 input structure
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: inputs
Not reported
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

missing or unspecified

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: metric implementation
Count of designs surviving the stage
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.metric_implementation

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: population
Yeast-display hits selected by the authors for follow-up
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.population

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: protocol id
uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.protocol_id

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison: split
Not reported
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

missing or unspecified

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison.split

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Origin
author_reported
Context-only references
De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

author reported

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.origin

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: uc-binder-20261022-eval-alphaproteo-s3-subcloned-expressed-il7ra

areas
proteins-complexes; protein-structure
contexts
research
comparison
adaptation: Not reported; aggregation: Count per target per stage; budget: Not reported; dataset version: IL-7RA, Table S1 input structure; inputs: Not reported; metric implementation: Count of designs surviving the stage; population: Yeast-display hits selected by the authors for follow-up; protocol id: uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel; split: Not reported
missing metadata
comparison.adaptation: note: No adaptation step.; reason: inapplicable; comparison.budget: note: Table S3 does not state how many of the target's yeast hits were available to select from.; reason: unreported; comparison.inputs: note: A funnel count, not a scored prediction.; reason: inapplicable; comparison.split: note: A design campaign, not a train/test split.; reason: inapplicable
origin
author_reported
protocol
uc-binder-20261022-protocol-alphaproteo-ecoli-htrf-funnel
review
artifact sha256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42; method: source-hash-verification; independent-cell-check; ai-assisted-source-review; method note: Re-downloaded with curl (HTTP 200, 12,333,400 bytes) and the SHA-256 recomputed and matched. Own text layer made with pdftotext -layout (poppler 26.08.0), reproducing the extraction pass's 2,811 lines. Table 1, Table S2, Table S3 and Sections S1.2.1, S2, S4.1 and S4.2 read cell by cell and compared with every record. Column assignment re-derived arithmetically rather than trusted.; note: Reviewed in docs/reviews/use-cases/binder-2026-10-22.md. Automated AI review of the source transcription only; it is not human scientific review and nothing here was reproduced experimentally.; retrieval url: https://arxiv.org/pdf/2409.08022v1; reviewed at: 2026-10-22T15:00:00Z; reviewer: claude; reviewer note: Claude review agent, independent of the extraction agent; no human review claimed
source locator
Table S3, row 'Successfully subcloned and expressed', column 'IL-7RA'
version
arXiv:2409.08022v1 Table S3
Related records

Suggest a correction