| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.59 Bray curtis dissimilarity (RPM-based abundance profile; mean over mock communities; ANI-capped truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 26 (base_tool 'Kraken2', rank 'ani_capped', truth_mode 'capped'), column 'bray_curtis' (cell E26) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.54 F1 (taxon detection; mean over mock communities; ANI-capped truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 26 (base_tool 'Kraken2', rank 'ani_capped', truth_mode 'capped'), column 'F1' (cell D26) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.353 Pearson (abundance profile against truth; mean over mock communities; ANI-capped truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 26 (base_tool 'Kraken2', rank 'ani_capped', truth_mode 'capped'), column 'pearson' (cell F26) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.572 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank family, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 6 (base_tool 'Kraken2', rank 'family', truth_mode 'strict'), column 'bray_curtis' (cell E6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.754 F1 (taxon detection; mean over six mock communities; rank family, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 6 (base_tool 'Kraken2', rank 'family', truth_mode 'strict'), column 'F1' (cell D6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.384 Pearson (abundance profile against truth; mean over six mock communities; rank family, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 6 (base_tool 'Kraken2', rank 'family', truth_mode 'strict'), column 'pearson' (cell F6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.557 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank genus, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 11 (base_tool 'Kraken2', rank 'genus', truth_mode 'strict'), column 'bray_curtis' (cell E11) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.682 F1 (taxon detection; mean over six mock communities; rank genus, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 11 (base_tool 'Kraken2', rank 'genus', truth_mode 'strict'), column 'F1' (cell D11) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.406 Pearson (abundance profile against truth; mean over six mock communities; rank genus, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 11 (base_tool 'Kraken2', rank 'genus', truth_mode 'strict'), column 'pearson' (cell F11) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.598 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank species, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 16 (base_tool 'Kraken2', rank 'species', truth_mode 'strict'), column 'bray_curtis' (cell E16) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.632 F1 (taxon detection; mean over six mock communities; rank species, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 16 (base_tool 'Kraken2', rank 'species', truth_mode 'strict'), column 'F1' (cell D16) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.365 Pearson (abundance profile against truth; mean over six mock communities; rank species, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 16 (base_tool 'Kraken2', rank 'species', truth_mode 'strict'), column 'pearson' (cell F16) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.594 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank subspecies, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 21 (base_tool 'Kraken2', rank 'subspecies', truth_mode 'strict'), column 'bray_curtis' (cell E21) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.612 F1 (taxon detection; mean over six mock communities; rank subspecies, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 21 (base_tool 'Kraken2', rank 'subspecies', truth_mode 'strict'), column 'F1' (cell D21) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.37 Pearson (abundance profile against truth; mean over six mock communities; rank subspecies, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 21 (base_tool 'Kraken2', rank 'subspecies', truth_mode 'strict'), column 'pearson' (cell F21) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 24 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'max_n_species' (cell F6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 0.000186% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'mean_fp_pct' (cell D6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 0.0000498% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'median_fp_pct' (cell C6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 6 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'median_n_species' (cell E6) |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026) | 0.0451 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.02256255452587475 to 0.08820897881741546. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.01575080088172684 Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 6 (base_tool 'Kraken2', read_pairs 10), column 'prob' (cell C6); interval columns 'asymp.LCL' and 'asymp.UCL' |
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| Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026) | Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026) | 0.589 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.4695359295150824 to 0.6991817422401287. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0596107597598103 Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceKraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 11 (base_tool 'Kraken2', read_pairs 100), column 'prob' (cell C11); interval columns 'asymp.LCL' and 'asymp.UCL' |
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