rewirebio.iobenchmarks
Dataset

Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)

Benchmark data constructed by the EsViritu authors.

Evaluation results

5 evaluations · 10 results. Different protocols are not a single leaderboard.

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Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.0117 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.00495878224532846 to 0.02719564873770782. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.005072199752721945

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 5 (base_tool 'Centrifuger', read_pairs 10), column 'prob' (cell C5); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.198 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.1247430998401599 to 0.2996024520084267. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.04452631953737853

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 10 (base_tool 'Centrifuger', read_pairs 100), column 'prob' (cell C10); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.974 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.9472888410150351 to 0.9877770210442621. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.009555569960517363

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 4 (base_tool 'EsViritu', read_pairs 10), column 'prob' (cell C4); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.999 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.9958883308387158 to 0.9997762702050332. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0007134219297489364

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 9 (base_tool 'EsViritu', read_pairs 100), column 'prob' (cell C9); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.0451 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.02256255452587475 to 0.08820897881741546. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.01575080088172684

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Kraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 6 (base_tool 'Kraken2', read_pairs 10), column 'prob' (cell C6); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.589 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.4695359295150824 to 0.6991817422401287. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0596107597598103

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Kraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 11 (base_tool 'Kraken2', read_pairs 100), column 'prob' (cell C11); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
1 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.9975626013544785 to 0.9999866586638343. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0002398938614360291

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Metabuli v1.2.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 7 (base_tool 'Metabuli', read_pairs 10), column 'prob' (cell C7); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
1 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.998667970267063 to 0.9999999447574935. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.00002209870476776148

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Metabuli v1.2.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 12 (base_tool 'Metabuli', read_pairs 100), column 'prob' (cell C12); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.000884 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.0002455506729957742 to 0.003174226166650207. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0005770437158860627

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Sylph v0.9.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 8 (base_tool 'Sylph', read_pairs 10), column 'prob' (cell C8); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.00347 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.001204394110956901 to 0.009960703944958654. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.001871995713381193

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Sylph v0.9.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 13 (base_tool 'Sylph', read_pairs 100), column 'prob' (cell C13); interval columns 'asymp.LCL' and 'asymp.UCL'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Research readiness

0 of 4 readiness checks met. These checks assess whether the evidence supports a reproducible investigation; a source-checked score alone does not meet them.

Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

Exact outcomes, predictions, identifiers and evaluator are connected.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified
  • Sample annotations are recorded: not yet verified
  • Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • A pinned run recipe exists: not yet verified
  • Compute requirements are estimated: not yet verified

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Independent validation data exist: not yet verified
  • Overlap with training data is checked: not yet verified

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Dataset and evaluation context

A dataset supplies biological observations. The evaluation protocol defines how those observations are split, used and scored.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

7 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Access
Zenodo 10.5281/zenodo.23043160 (stated in Code and data availability; not retrieved)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.access

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Population
Reads synthesised with bbtools randomreads.sh from seven genomes (four Genomoviridae, three Picornaviridae) at 75.91% to 95.62% ANI to their closest database reference, at 1, 10, 100 and 1,000 reads, ten permutations per depth
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.population

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Source location
Methods 'Positive controls'; Supplemental Table S25
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.source_locator

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Split
No split
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.split

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Version
As described in Tisza et al. 2026 v1
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.version

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Description
Benchmark data constructed by the EsViritu authors.
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: description

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Name
Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Methods 'Positive controls'; Supplemental Table S25

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

Audit details

Field: name

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-data-pos

areas
microbes-communities
contexts
research
version
As described in Tisza et al. 2026 v1
population
Reads synthesised with bbtools randomreads.sh from seven genomes (four Genomoviridae, three Picornaviridae) at 75.91% to 95.62% ANI to their closest database reference, at 1, 10, 100 and 1,000 reads, ten permutations per depth
split
No split
source locator
Methods 'Positive controls'; Supplemental Table S25
access
Zenodo 10.5281/zenodo.23043160 (stated in Code and data availability; not retrieved)
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