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Evaluation

EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

Published developer benchmark; transcribed, not reproduced.

Evaluation results

1 evaluation · 2 results. Different protocols are not a single leaderboard.

Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) · Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)

Sorted by Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configurationRecall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
EsViritu v1.3.1, headline setting (Tisza et al. 2026)Evaluation0.9740.999
All 2 result rows with coverage, uncertainty and sources
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Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.974 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.9472888410150351 to 0.9877770210442621. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.009555569960517363

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 4 (base_tool 'EsViritu', read_pairs 10), column 'prob' (cell C4); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
0.999 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
fraction · higher

Uncertainty: 95% CI 0.9958883308387158 to 0.9997762702050332. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0007134219297489364

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

rna-pathogen-20261011-tisza2026-protocol-pos

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 9 (base_tool 'EsViritu', read_pairs 100), column 'prob' (cell C9); interval columns 'asymp.LCL' and 'asymp.UCL'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

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Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

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Missing or unresolved evidence

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  • File checksums match the recorded files: not yet verified
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  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

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  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified
  • Sample annotations are recorded: not yet verified
  • Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • A pinned run recipe exists: not yet verified
  • Compute requirements are estimated: not yet verified

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Independent validation data exist: not yet verified
  • Overlap with training data is checked: not yet verified

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Evaluation procedure

Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)

Configuration
EsViritu v1.3.1, headline setting (Tisza et al. 2026)
Protocol
Positive-control virus detection by read depth (Tisza et al. 2026 Table S2)
Dataset
Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
origin
Author-reported evaluation
configuration
Preprint v1 as retrieved 2026-10-11
protocol id
rna-pathogen-20261011-tisza2026-protocol-pos
dataset version
Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
split
No split
population
Reads synthesised with bbtools randomreads.sh from seven genomes (four Genomoviridae, three Picornaviridae) at 75.91% to 95.62% ANI to their closest database reference, at 1, 10, 100 and 1,000 reads, ten permutations per depth
inputs
Synthetic read pairs (bbtools randomreads.sh)
adaptation
Headline setting: sr mode (minimap2 short-read preset)
metric implementation
A detection is scored when a tool reports the genome's family. Bias-reduced logistic regression (brglm2 AS_mixed), genome as a fixed block, EsViritu as the reference tool; estimated marginal means at 10 and 100 read pairs.
aggregation
Marginal mean over seven genomes at fixed depth
budget
Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split
No split
Adaptation
Headline setting: sr mode (minimap2 short-read preset)
Scoring implementation
A detection is scored when a tool reports the genome's family. Bias-reduced logistic regression (brglm2 AS_mixed), genome as a fixed block, EsViritu as the reference tool; estimated marginal means at 10 and 100 read pairs.

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

38 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Comparison: adaptation
Headline setting: sr mode (minimap2 short-read preset)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: adaptation
Headline setting: sr mode (minimap2 short-read preset)
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: aggregation
Marginal mean over seven genomes at fixed depth
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: aggregation
Marginal mean over seven genomes at fixed depth
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: budget
Not reported
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

missing or unspecified

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: budget
Not reported
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

missing or unspecified

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: dataset version
Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: dataset version
Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: inputs
Synthetic read pairs (bbtools randomreads.sh)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: inputs
Synthetic read pairs (bbtools randomreads.sh)
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S2, rows base_tool 'EsViritu'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

2 source records and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-eval-pos-esviritu

areas
microbes-communities
contexts
research
origin
author_reported
protocol
rna-pathogen-20261011-tisza2026-protocol-pos
version
Preprint v1 as retrieved 2026-10-11
comparison
protocol id: rna-pathogen-20261011-tisza2026-protocol-pos; dataset version: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026); split: No split; population: Reads synthesised with bbtools randomreads.sh from seven genomes (four Genomoviridae, three Picornaviridae) at 75.91% to 95.62% ANI to their closest database reference, at 1, 10, 100 and 1,000 reads, ten permutations per depth; inputs: Synthetic read pairs (bbtools randomreads.sh); adaptation: Headline setting: sr mode (minimap2 short-read preset); metric implementation: A detection is scored when a tool reports the genome's family. Bias-reduced logistic regression (brglm2 AS_mixed), genome as a fixed block, EsViritu as the reference tool; estimated marginal means at 10 and 100 read pairs.; aggregation: Marginal mean over seven genomes at fixed depth; budget: Not reported
source locator
Supplemental Table S2, rows base_tool 'EsViritu'
limitations
Run by the developers of EsViritu, who chose each comparator's parameter grid and headline setting.; All tools queried the same Virus Pathogen Database (v3.2.4b), so reference content is held constant; real-world tools are usually run with their own databases.
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