Positive-control virus detection by read depth (Tisza et al. 2026 Table S2); bioRxiv v1

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5 recorded evaluations, 10 metric rows. A comparison chart has not yet been validated for these results. The table retains the individual findings and their sources.

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All evaluations

5 evaluations · 10 results. Different protocols are not a single leaderboard.

Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) · Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)

Sorted by Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes)	Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes)
Metabuli v1.2.0, headline setting (Tisza et al. 2026)Evaluation	1 (best in column)	1 (best in column)
EsViritu v1.3.1, headline setting (Tisza et al. 2026)Evaluation	0.974	0.999
Kraken2 v2.17.1, headline setting (Tisza et al. 2026)Evaluation	0.0451	0.589
Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Evaluation	0.0117	0.198
Sylph v0.9.0, headline setting (Tisza et al. 2026)Evaluation	0.000884	0.00347

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Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.0117 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.00495878224532846 to 0.02719564873770782. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.005072199752721945 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 5 (base_tool 'Centrifuger', read_pairs 10), column 'prob' (cell C5); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.198 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.1247430998401599 to 0.2996024520084267. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.04452631953737853 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 10 (base_tool 'Centrifuger', read_pairs 100), column 'prob' (cell C10); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.974 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.9472888410150351 to 0.9877770210442621. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.009555569960517363 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 4 (base_tool 'EsViritu', read_pairs 10), column 'prob' (cell C4); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.999 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.9958883308387158 to 0.9997762702050332. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0007134219297489364 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 9 (base_tool 'EsViritu', read_pairs 100), column 'prob' (cell C9); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.0451 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.02256255452587475 to 0.08820897881741546. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.01575080088172684 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 6 (base_tool 'Kraken2', read_pairs 10), column 'prob' (cell C6); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.589 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.4695359295150824 to 0.6991817422401287. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0596107597598103 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 11 (base_tool 'Kraken2', read_pairs 100), column 'prob' (cell C11); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	1 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.9975626013544785 to 0.9999866586638343. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0002398938614360291 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 7 (base_tool 'Metabuli', read_pairs 10), column 'prob' (cell C7); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	1 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.998667970267063 to 0.9999999447574935. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.00002209870476776148 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 12 (base_tool 'Metabuli', read_pairs 100), column 'prob' (cell C12); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.000884 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.0002455506729957742 to 0.003174226166650207. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0005770437158860627 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 8 (base_tool 'Sylph', read_pairs 10), column 'prob' (cell C8); interval columns 'asymp.LCL' and 'asymp.UCL'
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026)	0.00347 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.001204394110956901 to 0.009960703944958654. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.001871995713381193 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 13 (base_tool 'Sylph', read_pairs 100), column 'prob' (cell C13); interval columns 'asymp.LCL' and 'asymp.UCL'

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Author-reported evaluations: 5

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2 source records and release history

EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes · Original source · bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Original source · bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)

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Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-protocol-pos

areas: microbes-communities
contexts: research
protocol: A detection is scored when a tool reports the genome's family. Bias-reduced logistic regression (brglm2 AS_mixed), genome as a fixed block, EsViritu as the reference tool; estimated marginal means at 10 and 100 read pairs.
version: bioRxiv v1
source locator: Methods 'Positive controls'; Supplemental Table S2 caption
limitations: Detection is scored at family rank only.; Model-estimated probabilities, not observed proportions; ANI is confounded with genome (one genome per ANI level).; Seven genomes from two families.; The S2 caption does not name the parameter setting; the Results tie the regression to the headline parameters, so the results are attached to the headline configurations.

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