| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.241 Bray curtis dissimilarity (RPM-based abundance profile; mean over mock communities; ANI-capped truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 25 (base_tool 'EsViritu', rank 'ani_capped', truth_mode 'capped'), column 'bray_curtis' (cell E25) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.922 F1 (taxon detection; mean over mock communities; ANI-capped truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 25 (base_tool 'EsViritu', rank 'ani_capped', truth_mode 'capped'), column 'F1' (cell D25) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.825 Pearson (abundance profile against truth; mean over mock communities; ANI-capped truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 25 (base_tool 'EsViritu', rank 'ani_capped', truth_mode 'capped'), column 'pearson' (cell F25) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.267 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank family, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 5 (base_tool 'EsViritu', rank 'family', truth_mode 'strict'), column 'bray_curtis' (cell E5) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.948 F1 (taxon detection; mean over six mock communities; rank family, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 5 (base_tool 'EsViritu', rank 'family', truth_mode 'strict'), column 'F1' (cell D5) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.831 Pearson (abundance profile against truth; mean over six mock communities; rank family, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 5 (base_tool 'EsViritu', rank 'family', truth_mode 'strict'), column 'pearson' (cell F5) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.27 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank genus, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 10 (base_tool 'EsViritu', rank 'genus', truth_mode 'strict'), column 'bray_curtis' (cell E10) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.929 F1 (taxon detection; mean over six mock communities; rank genus, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 10 (base_tool 'EsViritu', rank 'genus', truth_mode 'strict'), column 'F1' (cell D10) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.817 Pearson (abundance profile against truth; mean over six mock communities; rank genus, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 10 (base_tool 'EsViritu', rank 'genus', truth_mode 'strict'), column 'pearson' (cell F10) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.275 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank species, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 15 (base_tool 'EsViritu', rank 'species', truth_mode 'strict'), column 'bray_curtis' (cell E15) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.845 F1 (taxon detection; mean over six mock communities; rank species, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 15 (base_tool 'EsViritu', rank 'species', truth_mode 'strict'), column 'F1' (cell D15) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.776 Pearson (abundance profile against truth; mean over six mock communities; rank species, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 15 (base_tool 'EsViritu', rank 'species', truth_mode 'strict'), column 'pearson' (cell F15) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.382 Bray curtis dissimilarity (RPM-based abundance profile; mean over six mock communities; rank subspecies, strict truth) unitless · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 20 (base_tool 'EsViritu', rank 'subspecies', truth_mode 'strict'), column 'bray_curtis' (cell E20) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.671 F1 (taxon detection; mean over six mock communities; rank subspecies, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 20 (base_tool 'EsViritu', rank 'subspecies', truth_mode 'strict'), column 'F1' (cell D20) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) Dataset: Six mutated virus mock communities with bacterial and human background (Tisza et al. 2026) | 0.564 Pearson (abundance profile against truth; mean over six mock communities; rank subspecies, strict truth) unitless · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Mutated virus mock-community profiling against ground truth (Tisza et al. 2026 Table S9) rna-pathogen-20261011-tisza2026-protocol-mock Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S9, row 20 (base_tool 'EsViritu', rank 'subspecies', truth_mode 'strict'), column 'pearson' (cell F20) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 1 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'max_n_species' (cell F4) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 2.07e-7% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'mean_fp_pct' (cell D4) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 0% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_fp_pct' (cell C4) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026) | 0 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_n_species' (cell E4) |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026) | 0.974 Recall (model-estimated family-level detection probability at 10 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.9472888410150351 to 0.9877770210442621. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.009555569960517363 Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 4 (base_tool 'EsViritu', read_pairs 10), column 'prob' (cell C4); interval columns 'asymp.LCL' and 'asymp.UCL' |
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| Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026) | Protocol: Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) Dataset: Positive-control synthetic reads from seven divergent virus genomes (Tisza et al. 2026) | 0.999 Recall (model-estimated family-level detection probability at 100 read pairs; marginal mean over seven positive-control genomes) fraction · higher Uncertainty: 95% CI 0.9958883308387158 to 0.9997762702050332. Asymptotic interval from brglm2 estimated marginal means; standard error printed 0.0007134219297489364 Coverage: Not reported scored / Not reported eligible | Author-reported evaluation · Source checkedMethods, coverage and sourceEsViritu v1.3.1 on Positive-control virus detection by read depth (Tisza et al. 2026 Table S2) rna-pathogen-20261011-tisza2026-protocol-pos Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S2, row 9 (base_tool 'EsViritu', read_pairs 100), column 'prob' (cell C9); interval columns 'asymp.LCL' and 'asymp.UCL' |
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