Dataset

Seven pathogen-free negative-control read libraries (Tisza et al. 2026)

Benchmark data constructed by the EsViritu authors.

Evaluation results

5 evaluations · 20 results. Different protocols are not a single leaderboard.

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Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	2 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'max_n_species' (cell F5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0.000003% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'mean_fp_pct' (cell D5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'median_fp_pct' (cell C5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'median_n_species' (cell E5)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	1 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'max_n_species' (cell F4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	2.07e-7% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'mean_fp_pct' (cell D4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_fp_pct' (cell C4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_n_species' (cell E4)
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	24 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'max_n_species' (cell F6)
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0.000186% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'mean_fp_pct' (cell D6)
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0.0000498% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'median_fp_pct' (cell C6)
Configuration: Kraken2 v2.17.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	6 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Kraken2 v2.17.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 6 (base_tool 'Kraken2'), column 'median_n_species' (cell E6)
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	71 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 7 (base_tool 'Metabuli'), column 'max_n_species' (cell F7)
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0.0846% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 7 (base_tool 'Metabuli'), column 'mean_fp_pct' (cell D7)
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0.0217% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 7 (base_tool 'Metabuli'), column 'median_fp_pct' (cell C7)
Configuration: Metabuli v1.2.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	18 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Metabuli v1.2.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 7 (base_tool 'Metabuli'), column 'median_n_species' (cell E7)
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 8 (base_tool 'Sylph'), column 'max_n_species' (cell F8)
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	NA% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 8 (base_tool 'Sylph'), column 'mean_fp_pct' (cell D8)
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	NA% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 8 (base_tool 'Sylph'), column 'median_fp_pct' (cell C8)
Configuration: Sylph v0.9.0, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Sylph v0.9.0 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 8 (base_tool 'Sylph'), column 'median_n_species' (cell E8)

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7 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Access Zenodo 10.5281/zenodo.23043160 (stated in Code and data availability; not retrieved) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.access` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Population Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.population` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Source location Methods 'Negative controls'; Supplemental Table S16 Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.source_locator` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Split No split Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.split` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Version As described in Tisza et al. 2026 v1 Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.version` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Description Benchmark data constructed by the EsViritu authors. Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `description` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Name Seven pathogen-free negative-control read libraries (Tisza et al. 2026) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Methods 'Negative controls'; Supplemental Table S16 Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `name` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

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Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

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EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes · Original source · bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML

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Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-data-neg

areas: microbes-communities
contexts: research
version: As described in Tisza et al. 2026 v1
population: Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each
split: No split
source locator: Methods 'Negative controls'; Supplemental Table S16
access: Zenodo 10.5281/zenodo.23043160 (stated in Code and data availability; not retrieved)

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