rewirebio.iobenchmarks
Evaluation

Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

Published developer benchmark; transcribed, not reproduced.

Evaluation results

1 evaluation · 4 results. Different protocols are not a single leaderboard.

Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) · Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)

Sorted by False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) (lower is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configurationFalse positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries)False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries)False positives (false species reported; maximum over seven negative-control libraries)False positives (false species reported; median over seven negative-control libraries)
Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Evaluation0.000003%0%20
All 4 result rows with coverage, uncertainty and sources
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Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
2 False positives (false species reported; maximum over seven negative-control libraries)
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

rna-pathogen-20261011-tisza2026-protocol-neg

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'max_n_species' (cell F5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
0.000003% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries)
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

rna-pathogen-20261011-tisza2026-protocol-neg

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'mean_fp_pct' (cell D5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
0% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries)
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

rna-pathogen-20261011-tisza2026-protocol-neg

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'median_fp_pct' (cell C5)
Configuration: Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
0 False positives (false species reported; median over seven negative-control libraries)
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Centrifuger v1.1.1-r309 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

rna-pathogen-20261011-tisza2026-protocol-neg

Aggregation: Not reported

Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 5 (base_tool 'Centrifuger'), column 'median_n_species' (cell E5)

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Research readiness

0 of 4 readiness checks met. These checks assess whether the evidence supports a reproducible investigation; a source-checked score alone does not meet them.

Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

Exact outcomes, predictions, identifiers and evaluator are connected.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Metrics are recomputed from the saved predictions: not yet verified
  • Sample annotations are recorded: not yet verified
  • Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • A pinned run recipe exists: not yet verified
  • Compute requirements are estimated: not yet verified

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • File checksums match the recorded files: not yet verified
  • Predictions are matched to the right samples: not yet verified
  • Score meaning and direction are confirmed: not yet verified
  • Independent validation data exist: not yet verified
  • Overlap with training data is checked: not yet verified

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Evaluation procedure

False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

Configuration
Centrifuger v1.1.1-r309, headline setting (Tisza et al. 2026)
Protocol
False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset
Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
origin
Author-reported evaluation
configuration
Preprint v1 as retrieved 2026-10-11
protocol id
rna-pathogen-20261011-tisza2026-protocol-neg
dataset version
Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
split
No split
population
Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each
inputs
Short reads (ONT reads for one negative-control library)
adaptation
Headline setting: minimum-hit-length 100, minimum score 0
metric implementation
Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.
aggregation
Median, mean or maximum over seven libraries
budget
Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split
No split
Adaptation
Headline setting: minimum-hit-length 100, minimum score 0
Scoring implementation
Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

38 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Comparison: adaptation
Headline setting: minimum-hit-length 100, minimum score 0
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: adaptation
Headline setting: minimum-hit-length 100, minimum score 0
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: aggregation
Median, mean or maximum over seven libraries
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: aggregation
Median, mean or maximum over seven libraries
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: budget
Not reported
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

missing or unspecified

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: budget
Not reported
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

missing or unspecified

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: dataset version
Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: dataset version
Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: inputs
Short reads (ONT reads for one negative-control library)
Context-only references
EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Retrieved: 2026-10-11T21:39:50Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison: inputs
Short reads (ONT reads for one negative-control library)
Context-only references
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx)

Original source ↗

Supplemental Table S6, row base_tool 'Centrifuger'

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)
Retrieved: 2026-10-11T21:45:51Z

not individually reviewed

No individual claim review recorded

author reported

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

2 source records and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-eval-neg-centrifuger

areas
microbes-communities
contexts
research
origin
author_reported
protocol
rna-pathogen-20261011-tisza2026-protocol-neg
version
Preprint v1 as retrieved 2026-10-11
comparison
protocol id: rna-pathogen-20261011-tisza2026-protocol-neg; dataset version: Seven pathogen-free negative-control read libraries (Tisza et al. 2026); split: No split; population: Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each; inputs: Short reads (ONT reads for one negative-control library); adaptation: Headline setting: minimum-hit-length 100, minimum score 0; metric implementation: Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.; aggregation: Median, mean or maximum over seven libraries; budget: Not reported
source locator
Supplemental Table S6, row base_tool 'Centrifuger'
limitations
Run by the developers of EsViritu, who chose each comparator's parameter grid and headline setting.; All tools queried the same Virus Pathogen Database (v3.2.4b), so reference content is held constant; real-world tools are usually run with their own databases.
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