Evaluation

EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

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Evaluation results

1 evaluation · 4 results. Different protocols are not a single leaderboard.

Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) · Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)

Sorted by False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) (lower is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries)	False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries)	False positives (false species reported; maximum over seven negative-control libraries)	False positives (false species reported; median over seven negative-control libraries)
EsViritu v1.3.1, headline setting (Tisza et al. 2026)Evaluation	2.07e-7%	0%	1	0

All 4 result rows with coverage, uncertainty and sources

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	1 False positives (false species reported; maximum over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'max_n_species' (cell F4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	2.07e-7% False positive rate (percentage of individual reads falsely assigned; mean over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'mean_fp_pct' (cell D4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0% False positive rate (percentage of individual reads falsely assigned; median over seven negative-control libraries) percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_fp_pct' (cell C4)
Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)	Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)	0 False positives (false species reported; median over seven negative-control libraries) count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source EsViritu v1.3.1 on False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) rna-pathogen-20261011-tisza2026-protocol-neg Aggregation: Not reported Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Supplemental Table S6, row 4 (base_tool 'EsViritu'), column 'median_n_species' (cell E4)

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

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Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

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Evaluation procedure

False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)

Configuration: EsViritu v1.3.1, headline setting (Tisza et al. 2026)
Protocol: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Dataset: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)

origin: Author-reported evaluation
configuration: Preprint v1 as retrieved 2026-10-11
protocol id: rna-pathogen-20261011-tisza2026-protocol-neg
dataset version: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
split: No split
population: Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each
inputs: Short reads (ONT reads for one negative-control library)
adaptation: Headline setting: sr mode (minimap2 short-read preset)
metric implementation: Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.
aggregation: Median, mean or maximum over seven libraries
budget: Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split: No split
Adaptation: Headline setting: sr mode (minimap2 short-read preset)
Scoring implementation: Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.

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Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

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Evidence table

Inspect claims, sources and review details

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One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

38 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Comparison: adaptation Headline setting: sr mode (minimap2 short-read preset) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.adaptation` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: adaptation Headline setting: sr mode (minimap2 short-read preset) Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.adaptation` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: aggregation Median, mean or maximum over seven libraries Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.aggregation` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: aggregation Median, mean or maximum over seven libraries Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.aggregation` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: budget Not reported Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	missing or unspecified No individual claim review recorded author reported Audit details Field: `attributes.comparison.budget` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: budget Not reported Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	missing or unspecified No individual claim review recorded author reported Audit details Field: `attributes.comparison.budget` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: dataset version Seven pathogen-free negative-control read libraries (Tisza et al. 2026) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.dataset_version` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: dataset version Seven pathogen-free negative-control read libraries (Tisza et al. 2026) Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.dataset_version` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: inputs Short reads (ONT reads for one negative-control library) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.inputs` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: inputs Short reads (ONT reads for one negative-control library) Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ Supplemental Table S6, row base_tool 'EsViritu' Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.inputs` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

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Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

2 source records and release history

EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes · Original source · bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Original source · bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)

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Technical metadata and extraction receipts

Stable ID: rna-pathogen-20261011-tisza2026-eval-neg-esviritu

areas: microbes-communities
contexts: research
origin: author_reported
protocol: rna-pathogen-20261011-tisza2026-protocol-neg
version: Preprint v1 as retrieved 2026-10-11
comparison: protocol id: rna-pathogen-20261011-tisza2026-protocol-neg; dataset version: Seven pathogen-free negative-control read libraries (Tisza et al. 2026); split: No split; population: Human genome mock reads (chm13v2, 20,000,004 reads), mouse RNA, anaerobic digester (ONT), freshwater, marine water, hot springs and soil libraries; 1.56 to 90.6 million reads each; inputs: Short reads (ONT reads for one negative-control library); adaptation: Headline setting: sr mode (minimap2 short-read preset); metric implementation: Every virus assignment in a pathogen-free library is a false positive. False-positive individual-read percentage uses each library's total reads as the denominator; Sylph read percentages are unavailable.; aggregation: Median, mean or maximum over seven libraries; budget: Not reported
source locator: Supplemental Table S6, row base_tool 'EsViritu'
limitations: Run by the developers of EsViritu, who chose each comparator's parameter grid and headline setting.; All tools queried the same Virus Pathogen Database (v3.2.4b), so reference content is held constant; real-world tools are usually run with their own databases.

Related records

system: EsViritu v1.3.1, headline setting (Tisza et al. 2026)
assessment: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
data: Seven pathogen-free negative-control read libraries (Tisza et al. 2026)
evaluation: tisza2026 neg-esviritu-max-species
evaluation: tisza2026 neg-esviritu-mean-fp-pct
evaluation: tisza2026 neg-esviritu-median-fp-pct
evaluation: tisza2026 neg-esviritu-median-species

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