Configuration

Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)

Karius mcfDNA-Seq results interpreted with: all positive tests.

Overview

An explanatory profile has not yet been reviewed for this record.

Consult the linked sources for architecture or protocol details. Missing evidence is not evidence of a missing capability.

Evaluations and results

3 evaluations · 40 results. Different protocols are not a single leaderboard.

Sorted by True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Protocol and dataset	True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	Recall (episode-level predictive sensitivity; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)	Specificity (predictive specificity; any bacterium or yeast (Burkholderia, moulds, viruses and parasites excluded); control samples without fever or infection within 7 days)	Specificity (predictive specificity; common bloodstream infection pathogens; control samples without fever or infection within 7 days)	True negatives (negative control samples; any bacterium or yeast (Burkholderia, moulds, viruses and parasites excluded); control samples without fever or infection within 7 days)	True negatives (negative control samples; common bloodstream infection pathogens; control samples without fever or infection within 7 days)	Count (evaluable episodes; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	Count (evaluable episodes; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	Count (evaluable episodes; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)
Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)Evaluation	Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2), Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	65 (best in column)	73 (best in column)	64 (best in column)	72 (best in column)	61 (best in column)	68 (best in column)	81·3%	83·0%	83·1%	84·7%	81·3%	82·9%	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	80	88	77	85	75	82
Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)Evaluation	Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2), Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	42	44	42	44	38	40	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	51·9%	51·2%	53·8%	53·0%	51·4%	50·6%	Not reported	Not reported	Not reported	Not reported	81	86	78	83	74	79
Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)Evaluation	Predictive specificity in control samples (Wolf et al. 2026 Table 3), Control plasma samples without fever or infection (Wolf et al. 2026)	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported	82·7%	88·9%	134	144	Not reported	Not reported	Not reported	Not reported	Not reported	Not reported

All 40 result rows with coverage, uncertainty and sources

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Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	88 Count (evaluable episodes; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	73 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	83·0% Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 73.4 to 90.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	80 Count (evaluable episodes; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	65 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	81·3% Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 71.0 to 89.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	85 Count (evaluable episodes; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	72 True positives (episodes with all culture organisms detected; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	84·7% Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 75.3 to 91.6 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	77 Count (evaluable episodes; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	64 True positives (episodes with all culture organisms detected; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	83·1% Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 72.9 to 90.7 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	82 Count (evaluable episodes; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	68 True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	82·9% Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 73.0 to 90.3 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	75 Count (evaluable episodes; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	61 True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	81·3% Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 70.7 to 89.4 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	86 Count (evaluable episodes; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	44 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	51·2% Recall (episode-level predictive sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 40.1 to 62.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	81 Count (evaluable episodes; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	42 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	51·9% Recall (episode-level predictive sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 40.5 to 63.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Prediction (day −3 to −1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	83 Count (evaluable episodes; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-prediction Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Prediction (day −3 to −1)', block 'With logical imputation of missing values', column 'Evaluable episodes'

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Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Original source · The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)

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Stable ID: dna-pathogen-20261011-wolf2026-config-all

areas: microbes-communities
contexts: clinical_research
method types: conventional_pipeline
reported name: All positive tests
parameters: Any organism reported by the laboratory counts as positive.
foundation model eligible: false
access reuse: Commercial laboratory test; testing was provided by Karius as non-financial support
source locator: Methods 'Statistical analysis' paragraphs 3 and 5; Table 3 footnote
missing metadata: version: reason: unreported; note: No assay, pipeline or database version is given in the inspected text

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