Protocol

Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2)

Proportion of evaluable bloodstream infection episodes in which all organisms identified by blood culture were detected by mcfDNA-Seq in at least one sample from the day of or day after onset.

1 evaluation · 18 results

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1 recorded evaluation, 18 metric rows. A comparison chart has not yet been validated for these results. The table retains the individual findings and their sources.

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Results

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All evaluations

1 evaluation · 18 results. Different protocols are not a single leaderboard.

Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) · Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)

Sorted by Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)	Count (evaluable episodes; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture)	Count (evaluable episodes; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture)	Count (evaluable episodes; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture)	Count (evaluable episodes; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture)
Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)Evaluation	81·3%	83·0%	83·1%	84·7%	81·3%	82·9%	65	73	64	72	61	68	80	88	77	85	75	82

All 18 result rows with coverage, uncertainty and sources

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Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	88 Count (evaluable episodes; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	73 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	83·0% Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 73.4 to 90.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	80 Count (evaluable episodes; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	65 True positives (episodes with all culture organisms detected; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	81·3% Recall (episode-level diagnostic sensitivity; all bloodstream infection episodes; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 71.0 to 89.1 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'All bloodstream infection episodes (n=94)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	85 Count (evaluable episodes; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	72 True positives (episodes with all culture organisms detected; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	84·7% Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 75.3 to 91.6 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	77 Count (evaluable episodes; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	64 True positives (episodes with all culture organisms detected; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	83·1% Recall (episode-level diagnostic sensitivity; bacterial bloodstream infections; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 72.9 to 90.7 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Bacterial bloodstream infections (n=91)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	82 Count (evaluable episodes; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	68 True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	82·9% Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; with logical imputation of missing values; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 73.0 to 90.3 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'With logical imputation of missing values', column 'Sensitivity'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	75 Count (evaluable episodes; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) count · unknown Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Evaluable episodes'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	61 True positives (episodes with all culture organisms detected; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) count · higher Uncertainty: Not applicable: Count Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Positive'
Configuration: Karius mcfDNA-Seq, all positive tests (Wolf et al. 2026)	Protocol: Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) Dataset: Bloodstream infection episodes in children and young people with high-risk leukaemia (Wolf et al. 2026)	81·3% Recall (episode-level diagnostic sensitivity; common bloodstream infection pathogens; raw data; reference: organisms identified by blood culture) percent · higher Uncertainty: 95% CI 70.7 to 89.4 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) dna-pathogen-20261011-wolf2026-protocol-diagnosis Aggregation: Not reported Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Table 2, group 'Common bloodstream infection pathogens (n=87)', row 'Diagnosis (day 0 or 1)', block 'Raw data', column 'Sensitivity'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

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Karius mcfDNA-Seq (all positive tests) on Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2)

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Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Original source · The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)

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Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261011-wolf2026-protocol-diagnosis

areas: microbes-communities
contexts: clinical_research
protocol: Proportion of evaluable bloodstream infection episodes in which all organisms identified by blood culture were detected by mcfDNA-Seq in at least one sample from the day of or day after onset. Raw data and logical imputation of missing values are both reported; 95% CIs as printed. The laboratory was masked to sample status.
version: Table 2
source locator: Methods 'Outcomes' and 'Statistical analysis' paragraphs 2-5
limitations: Reference for sensitivity is blood culture, which does not identify every infection; polymicrobial episodes count as detected only if every culture organism is found.

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