Evaluation

Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026)

Published clinical comparison; transcribed, not reproduced.

Evaluation results

1 evaluation · 20 results. Different protocols are not a single leaderboard.

Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) · Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)

Sorted by Accuracy (patient level; all pneumonia; reference: final clinical diagnosis) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Accuracy (patient level; all pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; fungal pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; all pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; fungal pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Precision (patient level; all pneumonia; reference: final clinical diagnosis)	Precision (patient level; fungal pneumonia; reference: final clinical diagnosis)	Precision (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Precision (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Recall (patient level; all pneumonia; reference: final clinical diagnosis)	Recall (patient level; fungal pneumonia; reference: final clinical diagnosis)	Recall (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Recall (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Specificity (patient level; all pneumonia; reference: final clinical diagnosis)	Specificity (patient level; fungal pneumonia; reference: final clinical diagnosis)	Specificity (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Specificity (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)
Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026Evaluation	60.7%	73%	60.7%	82.8%	17.4%	71.4%	50.6%	85.2%	86.8%	90%	79.1%	64.3%	63.5%	21.9%	46.6%	36%	44.4%	98.8%	81.6%	94.8%

All 20 result rows with coverage, uncertainty and sources

Filter evaluations

Tested model or methodProtocolDatasetEvaluation setupMetricEvidence origin

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	60.7% Accuracy (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 51.79 to 68.87 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	17.4% Negative predictive value (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 9.09 to 30.72 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	86.8% Precision (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 77.45 to 92.69 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	63.5% Recall (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 53.88 to 72.08 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	44.4% Specificity (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 24.56 to 66.28 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	73% Accuracy (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 64.46 to 80.04 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	71.4% Negative predictive value (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 62.46 to 78.98 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	90% Precision (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 59.58 to 98.21 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	21.9% Recall (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 12.00 to 36.71 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	98.8% Specificity (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 93.33 to 99.78 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	60.7% Accuracy (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 51.79 to 68.87 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	50.6% Negative predictive value (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 39.84 to 61.37 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	79.1% Precision (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 64.79 to 88.58 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	46.6% Recall (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 35.59 to 57.90 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	81.6% Specificity (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 68.64 to 90.02 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	82.8% Accuracy (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 75.11 to 88.46 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	85.2% Negative predictive value (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 77.28 to 90.67 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	64.3% Precision (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 38.76 to 83.66 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	36% Recall (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 20.25 to 55.48 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	94.8% Specificity (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 88.50 to 97.78 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Research readiness

0 of 4 readiness checks met. These checks assess whether the evidence supports a reproducible investigation; a source-checked score alone does not meet them.

Readiness checks, gaps and artifacts

Release 2026-10-22-1b7cf027e4f6 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

Exact outcomes, predictions, identifiers and evaluator are connected.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
Metrics are recomputed from the saved predictions: not yet verified

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
Metrics are recomputed from the saved predictions: not yet verified
Sample annotations are recorded: not yet verified
Dependence between samples is assessed: not yet verified

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
A pinned run recipe exists: not yet verified
Compute requirements are estimated: not yet verified

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

No verified artifact manifest is linked to this exact record.
File checksums match the recorded files: not yet verified
Predictions are matched to the right samples: not yet verified
Score meaning and direction are confirmed: not yet verified
Independent validation data exist: not yet verified
Overlap with training data is checked: not yet verified

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Evaluation procedure

Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2)

Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026
Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2)
Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)

origin: Author-reported evaluation
configuration: Primary source as retrieved 2026-10-11
protocol id: dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis
dataset version: 122-patient severe pneumonia-associated sepsis cohort (Huang et al. 2026)
split: Single cohort
population: 122 patients; strata by pathogen class as printed
inputs: Blood (microbial cell-free DNA for the NGS assays)
adaptation: VAMNE Magnetic Pathogen DNA/RNA extraction; HieffNGS C37P4 One Pot cDNA&gDNA library; 4 h hybridisation with Geneplus probes (1,872 organisms, STable 1) on Gene+GIN96; Gene+Seq-100 at 5 M reads; Gene+P-Seq-Tool Kits; reportable at RPM >= 6 for common pathogens and >= 0.5 for fungi and mycobacteria
metric implementation: Patient-level sensitivity, specificity, PPV, NPV and accuracy against the final clinical diagnosis
aggregation: All patients in the stratum
budget: Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split: Single cohort
Adaptation: VAMNE Magnetic Pathogen DNA/RNA extraction; HieffNGS C37P4 One Pot cDNA&gDNA library; 4 h hybridisation with Geneplus probes (1,872 organisms, STable 1) on Gene+GIN96; Gene+Seq-100 at 5 M reads; Gene+P-Seq-Tool Kits; reportable at RPM >= 6 for common pathogens and >= 0.5 for fungi and mycobacteria
Scoring implementation: Patient-level sensitivity, specificity, PPV, NPV and accuracy against the final clinical diagnosis

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

19 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Comparison: adaptation VAMNE Magnetic Pathogen DNA/RNA extraction; HieffNGS C37P4 One Pot cDNA&gDNA library; 4 h hybridisation with Geneplus probes (1,872 organisms, STable 1) on Gene+GIN96; Gene+Seq-100 at 5 M reads; Gene+P-Seq-Tool Kits; reportable at RPM >= 6 for common pathogens and >= 0.5 for fungi and mycobacteria Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.adaptation` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: aggregation All patients in the stratum Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.aggregation` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: budget Not reported Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	missing or unspecified No individual claim review recorded author reported Audit details Field: `attributes.comparison.budget` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: dataset version 122-patient severe pneumonia-associated sepsis cohort (Huang et al. 2026) Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.dataset_version` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: inputs Blood (microbial cell-free DNA for the NGS assays) Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.inputs` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: metric implementation Patient-level sensitivity, specificity, PPV, NPV and accuracy against the final clinical diagnosis Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.metric_implementation` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: population 122 patients; strata by pathogen class as printed Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.population` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: protocol id dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.protocol_id` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison: split Single cohort Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.comparison.split` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Limitations 2 values The reference was established primarily from BALF mNGS and CMT results, so CMTs is scored against a reference that contains it. Two co-authors (Dejian Gu and Hao Liu) are affiliated with Geneplus-Beijing, which supplies the bstNGS probes and analysis kits; the article declares no competing interests. Context-only references	Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis Original source ↗ Table 2, rows 'Blood-bstNGS' Version: Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML Retrieved: 2026-10-11T22:07:38Z	not individually reviewed No individual claim review recorded author reported Audit details Field: `attributes.limitations` Source artifact SHA-256: `79b237d733d9ad3af33722c30013afb0539adf03098ca1e1c2f18344bcc4a67d` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release history

Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Original source · Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML

Download this release (gzip)

Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261011-huang2026-eval-bstngs

areas: microbes-communities
contexts: clinical_research
origin: author_reported
protocol: dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis
version: Primary source as retrieved 2026-10-11
comparison: protocol id: dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis; dataset version: 122-patient severe pneumonia-associated sepsis cohort (Huang et al. 2026); split: Single cohort; population: 122 patients; strata by pathogen class as printed; inputs: Blood (microbial cell-free DNA for the NGS assays); adaptation: VAMNE Magnetic Pathogen DNA/RNA extraction; HieffNGS C37P4 One Pot cDNA&gDNA library; 4 h hybridisation with Geneplus probes (1,872 organisms, STable 1) on Gene+GIN96; Gene+Seq-100 at 5 M reads; Gene+P-Seq-Tool Kits; reportable at RPM >= 6 for common pathogens and >= 0.5 for fungi and mycobacteria; metric implementation: Patient-level sensitivity, specificity, PPV, NPV and accuracy against the final clinical diagnosis; aggregation: All patients in the stratum; budget: Not reported
source locator: Table 2, rows 'Blood-bstNGS'
limitations: The reference was established primarily from BALF mNGS and CMT results, so CMTs is scored against a reference that contains it.; Two co-authors (Dejian Gu and Hao Liu) are affiliated with Geneplus-Beijing, which supplies the bstNGS probes and analysis kits; the article declares no competing interests.

Related records

system: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026
assessment: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2)
data: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)
evaluation: huang2026 all bstngs accuracy
evaluation: huang2026 all bstngs npv
evaluation: huang2026 all bstngs ppv
evaluation: huang2026 all bstngs sensitivity
evaluation: huang2026 all bstngs specificity
evaluation: huang2026 fungal bstngs accuracy
evaluation: huang2026 fungal bstngs npv
evaluation: huang2026 fungal bstngs ppv
evaluation: huang2026 fungal bstngs sensitivity
evaluation: huang2026 fungal bstngs specificity
evaluation: huang2026 gnb bstngs accuracy
evaluation: huang2026 gnb bstngs npv
evaluation: huang2026 gnb bstngs ppv
evaluation: huang2026 gnb bstngs sensitivity
evaluation: huang2026 gnb bstngs specificity
evaluation: huang2026 gpb bstngs accuracy
evaluation: huang2026 gpb bstngs npv
evaluation: huang2026 gpb bstngs ppv
evaluation: huang2026 gpb bstngs sensitivity
evaluation: huang2026 gpb bstngs specificity

Suggest a correction