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Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026

Blood-bstNGS as applied to the blood samples of the 122-patient cohort.

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Evaluations and results

1 evaluation · 20 results. Different protocols are not a single leaderboard.

Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) · Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)

Sorted by Accuracy (patient level; all pneumonia; reference: final clinical diagnosis) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configuration	Accuracy (patient level; all pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; fungal pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Accuracy (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; all pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; fungal pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Negative predictive value (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Precision (patient level; all pneumonia; reference: final clinical diagnosis)	Precision (patient level; fungal pneumonia; reference: final clinical diagnosis)	Precision (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Precision (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Recall (patient level; all pneumonia; reference: final clinical diagnosis)	Recall (patient level; fungal pneumonia; reference: final clinical diagnosis)	Recall (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Recall (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)	Specificity (patient level; all pneumonia; reference: final clinical diagnosis)	Specificity (patient level; fungal pneumonia; reference: final clinical diagnosis)	Specificity (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis)	Specificity (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis)
Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026Evaluation	60.7%	73%	60.7%	82.8%	17.4%	71.4%	50.6%	85.2%	86.8%	90%	79.1%	64.3%	63.5%	21.9%	46.6%	36%	44.4%	98.8%	81.6%	94.8%

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	60.7% Accuracy (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 51.79 to 68.87 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	17.4% Negative predictive value (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 9.09 to 30.72 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	86.8% Precision (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 77.45 to 92.69 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	63.5% Recall (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 53.88 to 72.08 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	44.4% Specificity (patient level; all pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 24.56 to 66.28 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'All pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	73% Accuracy (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 64.46 to 80.04 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	71.4% Negative predictive value (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 62.46 to 78.98 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	90% Precision (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 59.58 to 98.21 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	21.9% Recall (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 12.00 to 36.71 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	98.8% Specificity (patient level; fungal pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 93.33 to 99.78 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'Fungal pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	60.7% Accuracy (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 51.79 to 68.87 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	50.6% Negative predictive value (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 39.84 to 61.37 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	79.1% Precision (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 64.79 to 88.58 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	46.6% Recall (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 35.59 to 57.90 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	81.6% Specificity (patient level; gram-negative bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 68.64 to 90.02 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GNB pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	82.8% Accuracy (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 75.11 to 88.46 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Accuracy (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	85.2% Negative predictive value (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 77.28 to 90.67 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'NPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	64.3% Precision (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 38.76 to 83.66 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'PPV (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	36% Recall (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 20.25 to 55.48 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Sensitivity (95%CI)'
Configuration: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS), as run in Huang et al. 2026	Protocol: Patient-level detection of causative pathogens in blood against the final clinical diagnosis (Huang et al. 2026 Table 2) Dataset: 122 ICU patients with severe pneumonia-associated sepsis and paired blood and BALF (Huang et al. 2026)	94.8% Specificity (patient level; gram-positive bacterial pneumonia; reference: final clinical diagnosis) percent · higher Uncertainty: 95% CI 88.50 to 97.78 Coverage: Not reported scored / Not reported eligible	Author-reported evaluation · Source checked Methods, coverage and source Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026) dna-pathogen-20261011-huang2026-protocol-clinical-diagnosis Aggregation: Not reported Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Table 2, stratum 'GPB pneumonia', row 'Blood-bstNGS', column 'Specificity (95%CI)'

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Blood-based targeted sequencing of microbial cell-free DNA in severe pneumonia-associated sepsis · Original source · Respiratory Research 27:357, published 2026-06-24; PMC13584460 full-text XML

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Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261011-huang2026-config-bstngs

areas: microbes-communities
contexts: clinical_research
method types: conventional_pipeline
reported name: Blood-bstNGS
parameters: VAMNE Magnetic Pathogen DNA/RNA extraction; HieffNGS C37P4 One Pot cDNA&gDNA library; 4 h hybridisation with Geneplus probes (1,872 organisms, STable 1) on Gene+GIN96; Gene+Seq-100 at 5 M reads; Gene+P-Seq-Tool Kits; reportable at RPM >= 6 for common pathogens and >= 0.5 for fungi and mycobacteria
foundation model eligible: false
access reuse: Commercial assay; one co-author is affiliated with Geneplus-Beijing
source locator: Methods 'bstNGS assay workflow and mNGS steps'; Supplementary Methods (not inspected)
missing metadata: version: reason: unreported; note: No software or database versions are given

Related records

configuration of: Geneplus broad-spectrum targeted NGS of microbial cell-free DNA (bstNGS)
system: Blood-bstNGS against the final clinical diagnosis (Huang et al. 2026)

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