Nasal washings from COPD patients with respiratory complaints, metagenomic sequencing and 13-target respiratory PCR panel (Carbo et al. 2022)
88 clinical metagenomic datasets with 1144 PCR results as reference.
Research readiness
These checks assess whether the evidence supports a reproducible investigation. A source-checked score alone does not meet these requirements.
Release 2026-10-09-ba02f2f4a36e · Evidence verified: Not verified
Evidence incomplete
Replay metrics
Exact outcomes, predictions, identifiers and evaluator are connected.
Missing or unresolved evidence
No verified artifact manifest is linked to this exact record.
artifact hashes: verification is missing
join integrity: verification is missing
score semantics: verification is missing
metric replay: verification is missing
Verified: Not verified
Evidence incomplete
Investigate discrepancies
Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.
Missing or unresolved evidence
No verified artifact manifest is linked to this exact record.
artifact hashes: verification is missing
join integrity: verification is missing
score semantics: verification is missing
metric replay: verification is missing
annotations: verification is missing
dependence: verification is missing
Verified: Not verified
Evidence incomplete
Run locally
A pinned recipe describes the inputs, environment and resource requirements.
Missing or unresolved evidence
No verified artifact manifest is linked to this exact record.
artifact hashes: verification is missing
join integrity: verification is missing
score semantics: verification is missing
recipe pinned: verification is missing
resource estimate: verification is missing
Verified: Not verified
Evidence incomplete
Validate independently
Separate data and exposure records support an independent test.
Missing or unresolved evidence
No verified artifact manifest is linked to this exact record.
artifact hashes: verification is missing
join integrity: verification is missing
score semantics: verification is missing
independent validation: verification is missing
overlap checked: verification is missing
Verified: Not verified
Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.
Artifacts and reproduction
No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.
Source checking is not independent reproduction. Release 2026-10-09-ba02f2f4a36e.
Dataset and evaluation context
A dataset supplies biological observations. The evaluation protocol defines how those observations are split, used and scored.
Evidence
Source checking verifies the cited claim or transcription. It does not establish independent reproduction.
Evidence table
Inspect claims, sources and review details
Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.
One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.
9 evidence rows matching the loaded filters
Claims, original sources and review scope · Release 2026-10-09-ba02f2f4a36e
EAV and PhHV-1 internal controls; MagNAPure 96 total nucleic acid extraction; NEBNext Ultra II Directional RNA library with a protocol for RNA and DNA in one tube; NextSeq 500, about 10 million 150 bp paired-end reads per sample
88 nasal washings from 63 patients with COPD suspected of respiratory infection; 13 respiratory virus PCR targets per sample, 24 positive and 1120 negative PCR results. All targets are RNA viruses (rhinovirus/enterovirus, parainfluenza 1-4, influenza A and B, coronaviruses NL63, 229E, HKU1/OC43, metapneumovirus, RSV).
As described in Carbo et al. 2022 (NCBI SRA SRX6713943-SRX6714030, human reads removed)
accession
NCBI SRA SRX6713943-SRX6714030
population
88 nasal washings from 63 patients with COPD suspected of respiratory infection; 13 respiratory virus PCR targets per sample, 24 positive and 1120 negative PCR results. All targets are RNA viruses (rhinovirus/enterovirus, parainfluenza 1-4, influenza A and B, coronaviruses NL63, 229E, HKU1/OC43, metapneumovirus, RSV).
assay
EAV and PhHV-1 internal controls; MagNAPure 96 total nucleic acid extraction; NEBNext Ultra II Directional RNA library with a protocol for RNA and DNA in one tube; NextSeq 500, about 10 million 150 bp paired-end reads per sample
split
No split; whole cohort
denominator
1144
source locator
Sections 2.1-2.2; Table 1; Data Availability Statement