Minigene reporter screen of mutagenised variants in the 265 nt upstream of DNM1 exon 10a, assayed in five cell line and promoter combinations, with variants called active when they shift 3' splice site use.
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5 evidence rows matching the loaded filters
| Property and statement | Original source and location | Review and provenance |
|---|---|---|
| Assay Barcoded minigene splicing reporter, amplicon RNA-seq, three biological replicates; HEK293, KELLY, T47D, A172, DAOY or GAMG cells (five passing cell line and promoter combinations) Context-only references | AlphaGenome Atlas: in silico mutagenesis of the entire human genome (Cheng, Taylor, Nicolaisen et al., medRxiv 2026) Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Version: medRxiv preprint version 1, posted 2026-09-20; full-text PDF with supplementary figures and tables (83 pages) | not individually reviewed No individual claim review recorded Audit detailsField: Source artifact SHA-256: Hash scope: SHA-256 of one retrieval of the medRxiv PDF. A second retrieval at 2026-10-19T12:30:54Z had the same length (15,415,633 bytes) and the same SHA-256. |
| Label semantics Active: mean alternative 3' splice site rate above 0.10 across passing conditions and dominant alternative site coherence at least 0.5. 12 intronic variants produced in-frame exon extensions. Context-only references | AlphaGenome Atlas: in silico mutagenesis of the entire human genome (Cheng, Taylor, Nicolaisen et al., medRxiv 2026) Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Version: medRxiv preprint version 1, posted 2026-09-20; full-text PDF with supplementary figures and tables (83 pages) | not individually reviewed No individual claim review recorded Audit detailsField: Source artifact SHA-256: Hash scope: SHA-256 of one retrieval of the medRxiv PDF. A second retrieval at 2026-10-19T12:30:54Z had the same length (15,415,633 bytes) and the same SHA-256. |
| Source location Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Context-only references | AlphaGenome Atlas: in silico mutagenesis of the entire human genome (Cheng, Taylor, Nicolaisen et al., medRxiv 2026) Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Version: medRxiv preprint version 1, posted 2026-09-20; full-text PDF with supplementary figures and tables (83 pages) | not individually reviewed No individual claim review recorded Audit detailsField: Source artifact SHA-256: Hash scope: SHA-256 of one retrieval of the medRxiv PDF. A second retrieval at 2026-10-19T12:30:54Z had the same length (15,415,633 bytes) and the same SHA-256. |
| Description Minigene reporter screen of mutagenised variants in the 265 nt upstream of DNM1 exon 10a, assayed in five cell line and promoter combinations, with variants called active when they shift 3' splice site use. Context-only references | AlphaGenome Atlas: in silico mutagenesis of the entire human genome (Cheng, Taylor, Nicolaisen et al., medRxiv 2026) Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Version: medRxiv preprint version 1, posted 2026-09-20; full-text PDF with supplementary figures and tables (83 pages) | not individually reviewed No individual claim review recorded Audit detailsField: Source artifact SHA-256: Hash scope: SHA-256 of one retrieval of the medRxiv PDF. A second retrieval at 2026-10-19T12:30:54Z had the same length (15,415,633 bytes) and the same SHA-256. |
| Name DNM1 intron saturation mutagenesis minigene splicing screen (AlphaGenome Atlas Data S1) Context-only references | AlphaGenome Atlas: in silico mutagenesis of the entire human genome (Cheng, Taylor, Nicolaisen et al., medRxiv 2026) Methods 'Experimental splicing screen of DNM1 intron' (pages 43 to 44); Results paragraph on DNM1 (page 8) Version: medRxiv preprint version 1, posted 2026-09-20; full-text PDF with supplementary figures and tables (83 pages) | not individually reviewed No individual claim review recorded Audit detailsField: Source artifact SHA-256: Hash scope: SHA-256 of one retrieval of the medRxiv PDF. A second retrieval at 2026-10-19T12:30:54Z had the same length (15,415,633 bytes) and the same SHA-256. |
Release 2026-11-02-a7f03c9e55a5 · Record review: source checked
Stable ID: ag-atlas-20261019-data-dnm1-intron-screen