Protocol

ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)

Presence or absence of each mock community species from the method's species-level read counts.

Overview

Presence or absence of each mock community species from the method's species-level read counts.

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14 recorded evaluations, 112 metric rows. A comparison chart has not yet been validated for these results. The table retains the individual findings and their sources.

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Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Original source · BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML

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Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species

areas: microbes-communities
contexts: clinical_research
protocol: A species is detected when its cumulative read count exceeds 0.001% of total reads (20 reads for this dataset). True positive: a mock community species detected; false positive: a detected species not in the community; false negative: a community species not detected. Precision, recall, F1 and F0.5 from these counts. L1: sum of absolute differences between estimated and theoretical species percent abundances, with all false positives pooled as 'Other' against a theoretical 0.
version: Table 4 block 'ONT Q20 Zymo D6300 (10 species, even)'
source locator: Methods 'Detection metrics' paragraphs 1-4; 'Relative abundance estimates' paragraph 4; Table 3 (threshold read counts)
limitations: Mock community of cultured organisms; no human host DNA, no clinical specimen matrix.; Detection threshold is a fixed fraction of total reads, which penalises methods that assign fewer reads (Methods 'Detection metrics' paragraph 1).; Each method used its own reference database.
missing metadata: uncertainty: reason: unreported; note: Single run per dataset; no intervals printed

Related records

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Exact evaluated configurations and original reported results
Tested configuration	Protocol and dataset	Finding	Evidence and details
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.06 f-beta-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'F0.5'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.1 f1-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'F1'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0 false-negative-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'False negatives'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	184 false-positive-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'False positives'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	17% composition-l1-distance percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'L1'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.05 precision fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'Precision'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	1 recall fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'Recall'
Configuration: Bracken (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	10 true-positive-count count · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Bracken on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Bracken' (table row 52), column 'True positives'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.93 f-beta-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'F0.5'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.95 f1-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'F1'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0 false-negative-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'False negatives'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	1 false-positive-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'False positives'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	12.6% composition-l1-distance percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'L1'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.91 precision fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'Precision'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	1 recall fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'Recall'
Configuration: BugSeq-V2 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	10 true-positive-count count · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source BugSeq-V2 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'BugSeq-V2' (table row 64), column 'True positives'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.18 f-beta-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'F0.5'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.25 f1-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'F1'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	2 false-negative-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'False negatives'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	45 false-positive-count count · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'False positives'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	30.7% composition-l1-distance percent · lower Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'L1'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.15 precision fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'Precision'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.8 recall fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'Recall'
Configuration: Centrifuge-h22 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	8 true-positive-count count · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h22 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 53), column 'True positives'
Configuration: Centrifuge-h500 (Portik et al. 2022)	Protocol: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4) Dataset: ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION)	0.44 f-beta-score fraction · higher Uncertainty: Not reported by the source Coverage: Not reported scored / Not reported eligible	Independent external evaluation · Source checked Methods, coverage and source Centrifuge-h500 on ONT Q20 ZymoBIOMICS D6300 standard, length-filtered (PromethION) dna-pathogen-20261009-protocol-portik2022-ont-q20-zymo-d6300-species Aggregation: Not reported Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT Q20 Zymo D6300 (10 species, even)', row 'Centrifuge-h500' (table row 54), column 'F0.5'