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MetaPhlAn3

MetaPhlAn3 is the marker-based metagenomic comparator in the NABAS+ study.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 4); Introduction (paragraph 4)

1 evaluation · 1 result

How it worksEvaluated procedure (conceptual)
Evaluated procedure (conceptual)1. Shotgun metagenomic reads and a versioned marker database. Then: 2. MetaPhlAn3. Then: 3. Taxonomic abundance profilesEvaluated procedure (conceptual)1. Shotgun metagenomic reads and a versioned marker database. Then: 2. MetaPhlAn3. Then: 3. Taxonomic abundance profilesEvaluated procedure (conceptual)1. Shotgun metagenomic reads and a versioned marker database. Then: 2. MetaPhlAn3. Then: 3. Taxonomic abundance profiles

Conceptual input–method–output guide. Check the procedure text and linked evaluation for fitted components, additional inputs and exact settings.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)

Overview

Model type

Marker-based taxonomic profiler; this record is the paper-specific evaluated configuration.

Sourcesbiobakery/MetaPhlAn README.md · README.md model description

limited source coverage · Automated source review, 2026-09-16. All specifications and missing details

Evaluations and results

1 evaluation · 1 result. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: MetaPhlAn3Task: Metagenomic taxonomic classification
Dataset: CAMI II Toy human gastrooral sample19-new
0.753 F1 score
unitless · unknown

Uncertainty: Not reported

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

MetaPhlAn3: Metagenomic taxonomic classification

Newly generated sample19 used for classifier comparison.

Aggregation: Not reported

Advancing metagenomic classification with NABAS+: a novel alignment-based approach · Table 3, Sample19-new / MetaPhlAn3 row, F1 score column

Source checking is not independent reproduction. Release 2026-09-29-06401fd5b220.

Use this model

How it works, versions and access

How it works

How the evaluated method works

Taxonomic profiling uses clade-specific marker information to estimate microbial composition from shotgun reads.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)
Underlying method and version boundaries

MetaPhlAn profiles taxonomic composition using a marker reference. The repository’s current release is not the historical MetaPhlAn3 configuration; software and database versions require separate identification.

Sourcesbiobakery/MetaPhlAn README.md · README.md; introduction, model description, pretrained-model and usage sections at pinned revision
What was evaluated

The linked evaluation record identifies MetaPhlAn3: Metagenomic taxonomic classification. Its dataset, split, adaptation and evidence origin remain attached to the reported results.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · The named evaluation’s methods and comparison table; exact preserved evaluation IDs: evaluation-lit-040
Strengths, limitations and unresolved questions

Strengths and limitations

Limitations and conditions

Profile review details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Stable record: reported-model-df4084611520b7

Specifications

Inputs, training, access and other details

Explanatory profile: limited source coverage · Automated source review, 2026-09-16. Review applies to the cited claims; unresolved fields are listed below. Numerical results retain their own review status.

Inputs, outputs and configuration
PropertyDescription and evidence
Model typeMarker-based taxonomic profiler; this record is the paper-specific evaluated configuration.
Sourcesbiobakery/MetaPhlAn README.md · README.md model description
Architecture / procedureTaxonomic profiling uses clade-specific marker information to estimate microbial composition from shotgun reads.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)
Biological inputsShotgun metagenomic reads and a versioned marker database
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Discussion (paragraph 6); Introduction (paragraph 3)
OutputsTaxonomic abundance profiles
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 8); Materials and methods/Datasets and reference databases/Recreating the CAMI sample19 (paragraph 1)
ParametersNot applicable: taxonomic marker profiling does not have a pretrained neural parameter total. · Not applicable
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Selecting and setting up reference classifiers for NABAS+ benchmarking (paragraph 2); Materials and methods/Running the classifiers (paragraph 2)
Known versions / configurationMetaPhlAn3, as named in the paper; no exact package/database hash is supplied by the catalogue row. · Not reported in inspected sources
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Model identification in the comparison table and corresponding Methods; immutable checkpoint revision is not supplied by the table label.
Training data / fittingReference-marker classification without task-specific neural fitting. CAMI comparisons use mpa_v31_CHOCOPhlAn_201901; a newer Zymo comparison uses mpa_vOct22_CHOCOPhlAnSGB_202212. These database conditions are not pooled.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods / Datasets and reference databases / CAMI samples and Zymo community standards, database lists
Context limitsNot applicable to a pretrained sequence-token window; read/contig lengths and the reference-database or comparison configuration determine the analysed input. · Not applicable
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Discussion (paragraph 6)
AccessOfficial upstream implementation and usage documentation: https://github.com/biobakery/MetaPhlAn/blob/424f3e6e30618266404353e1083c6405a9f02f48/README.md. This pinned documentation revision is not automatically the evaluated weight revision.
Sourcesbiobakery/MetaPhlAn README.md · README.md; installation, model download and usage instructions
Code licenceRepository-specific licence text is available; a standard SPDX label has not been established. (upstream repository code at the cited revision; this does not establish every dependency or historical checkpoint licence).
Sourcesbiobakery/MetaPhlAn license.txt · license.txt; complete licence text
Weights licenceNot applicable to neural weights; the software and reference database have separate reuse terms. · Not applicable
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Datasets and reference databases/CAMI samples (paragraph 2)

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-09-29-06401fd5b220
Property and statementOriginal source and locationReview and provenance
Diagram caption
Conceptual input–method–output guide. Check the procedure text and linked evaluation for fitted components, additional inputs and exact settings.
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.caption

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram steps
  • Shotgun metagenomic reads and a versioned marker database
  • MetaPhlAn3
  • Taxonomic abundance profiles
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.steps

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram title
Evaluated procedure (conceptual)
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.title

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Model type
Marker-based taxonomic profiler; this record is the paper-specific evaluated configuration.
Individual claims
biobakery/MetaPhlAn README.md

Original source ↗

README.md model description

Version: 424f3e6e30618266404353e1083c6405a9f02f48
Retrieved: 2026-09-16T20:00:02.332125+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.0.value

Source artifact SHA-256: ce491bb2d686145e0773c685d0d02e8a5fabc7daaea60eef07cf54298561fba7

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Architecture / procedure
Taxonomic profiling uses clade-specific marker information to estimate microbial composition from shotgun reads.
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.1.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Weights licence
Not applicable to neural weights; the software and reference database have separate reuse terms.
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Datasets and reference databases/CAMI samples (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

inapplicable

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.10.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Biological inputs
Shotgun metagenomic reads and a versioned marker database
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Discussion (paragraph 6); Introduction (paragraph 3)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.2.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Outputs
Taxonomic abundance profiles
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 8); Materials and methods/Datasets and reference databases/Recreating the CAMI sample19 (paragraph 1)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.3.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Parameters
Not applicable: taxonomic marker profiling does not have a pretrained neural parameter total.
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Selecting and setting up reference classifiers for NABAS+ benchmarking (paragraph 2); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

inapplicable

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Known versions / configuration
MetaPhlAn3, as named in the paper; no exact package/database hash is supplied by the catalogue row.
Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Model identification in the comparison table and corresponding Methods; immutable checkpoint revision is not supplied by the table label.

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

unreported

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.5.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

View linked audit checks and correction history

Release 2026-09-29-06401fd5b220 · Record review: needs review

3 source records and release historyDownload this release
Technical metadata and extraction receipts

Stable ID: reported-model-df4084611520b7

areas
microbes-communities
entity level
method
version
Not reported
reported name
MetaPhlAn3
historical missing metadata
version: not_reported_in_legacy_extract; checkpoint revision: not_reported_in_legacy_extract; training data: not_reported_in_legacy_extract; licence: not_reported_in_legacy_extract
metadata review scope
historical_missing_metadata preserves the original discovery state. Current descriptive evidence and missingness are recorded in profile.facts; numerical-result review is separate.
legacy kinds
model
entity classification
review date: 2026-09-17; rationale: This source-scoped entry preserves the method/configuration actually named in an evaluation. It is neither a global family identity nor proof of an immutable checkpoint; the linked evaluation retains adaptation, fitting and scoring details.; source ids: nabas-plus-2025; evidence-reported-base-metaphlan-readme-md; source locator: Materials and methods/Datasets and reference databases/Consent for sample collection (paragraph 1); Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 6) | README.md model description | Results/Creating a novel alignment-based metagenomic tool to improve classification accuracy and reduce false positives (paragraph 4); Introduction (paragraph 4); ambiguities: Configuration means the source-labelled evaluated identity. It does not establish missing checkpoint hashes, default settings or equivalence to same-named records in other papers.
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