Evidence claim

Relevance of False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6) to "Select an RNA pathogen-detection workflow for diagnostic testing"

Use case: Select an RNA pathogen-detection workflow for diagnostic testing
Assessed by: False positives in seven pathogen-free libraries (Tisza et al. 2026 Table S6)
Relevance: Proxy evidence
Endpoint: False-positive read percentage and false species counts in seven pathogen-free libraries, including human genome mock reads and mouse RNA

False assignments in pathogen-free libraries, including human genome reads and mouse RNA, bear directly on the use case's question of distinguishing real detections from host or background signal. It is proxy evidence: no clinical specimens, and the libraries are not RNA-virus diagnostic samples.

5 reviewed evaluations

Evidence

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Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

40 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Citation locations 2 values source id rna-pathogen-20261011-tisza2026-source locator Methods 'Tools and parameters', 'Positive controls', 'Negative controls', 'Mock communities' source id rna-pathogen-20261011-tisza2026-source-supp locator Supplemental Table S6 Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.citation_locators` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Citation locations 2 values source id rna-pathogen-20261011-tisza2026-source locator Methods 'Tools and parameters', 'Positive controls', 'Negative controls', 'Mock communities' source id rna-pathogen-20261011-tisza2026-source-supp locator Supplemental Table S6 Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.citation_locators` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison group tisza2026-virus-profilers Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_group` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison group tisza2026-virus-profilers Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_group` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison title Virus profilers on a shared Virus Pathogen Database (Tisza et al. 2026, developer benchmark) Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_title` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison title Virus profilers on a shared Virus Pathogen Database (Tisza et al. 2026, developer benchmark) Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_title` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Constraints 2 values Inspect every linked evaluation's source locator and preserved limitations before citing a result. Do not combine these results with another protocol's results; datasets, databases and scoring rules differ between sources. Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.constraints` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Constraints 2 values Inspect every linked evaluation's source locator and preserved limitations before citing a result. Do not combine these results with another protocol's results; datasets, databases and scoring rules differ between sources. Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.constraints` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Endpoint False-positive read percentage and false species counts in seven pathogen-free libraries, including human genome mock reads and mouse RNA Context-only references	EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML Retrieved: 2026-10-11T21:39:50Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.endpoint` Source artifact SHA-256: `fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Endpoint False-positive read percentage and false species counts in seven pathogen-free libraries, including human genome mock reads and mouse RNA Context-only references	Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) Original source ↗ rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6 Shared locator for this statement’s cited sources; not a separate locator for each citation. Version: bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx) Retrieved: 2026-10-11T21:45:51Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.endpoint` Source artifact SHA-256: `0559022a3ff5ef5b2682e3c451eda51835c4c8d9be9dfdb1ebc9cd9295fb7701` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

2 source records and release history

EsViritu is a sensitive and specific profiler for viral pathogen detection and genome recovery in metagenomes · Original source · bioRxiv 10.64898/2026.09.29.755457 v1, posted 2026-09-30; JATS XML
Tisza et al. 2026 Supplemental Tables S1-S25 (bioRxiv media-1.xlsx) · Original source · bioRxiv 10.64898/2026.09.29.755457 v1 supplementary file DC1 (755457_file02.xlsx)

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Technical metadata and extraction receipts

Stable ID: use-case-mapping-rna-pathogen-20261011-tisza2026-neg

field: links:assessed_by:rna-pathogen-20261011-tisza2026-protocol-neg
value: rna-pathogen-20261011-tisza2026-protocol-neg
relevance: proxy
endpoint: False-positive read percentage and false species counts in seven pathogen-free libraries, including human genome mock reads and mouse RNA
rationale: False assignments in pathogen-free libraries, including human genome reads and mouse RNA, bear directly on the use case's question of distinguishing real detections from host or background signal. It is proxy evidence: no clinical specimens, and the libraries are not RNA-virus diagnostic samples.
constraints: Inspect every linked evaluation's source locator and preserved limitations before citing a result.; Do not combine these results with another protocol's results; datasets, databases and scoring rules differ between sources.
limitations: Developer benchmark: the EsViritu authors built the data, chose the comparators' parameter grids and headline settings, and ran every tool (author_reported).; Preprint (bioRxiv v1); not peer reviewed.; Synthetic reads or pathogen-free libraries only; no clinical specimens and no reference laboratory test.; All tools queried the same Virus Pathogen Database (v3.2.4b), so results compare algorithms with reference content held constant, not tools as usually deployed with their own databases.; Only one of the seven libraries is RNA-derived (mouse RNA).
citation locators: source id: rna-pathogen-20261011-tisza2026-source; locator: Methods 'Tools and parameters', 'Positive controls', 'Negative controls', 'Mock communities'; source id: rna-pathogen-20261011-tisza2026-source-supp; locator: Supplemental Table S6
source locator: rna-pathogen-20261011-tisza2026-source-supp: Supplemental Table S6
revision: 1
reason: Recorded from the 2026-10-11 pathogen-detection literature pass (data/omics/use-case-coverage-rna-pathogen-20261011-tisza2026). Independently reviewed in docs/reviews/use-cases/pathogen-use-cases-2026-10-11.md.
comparison group: tisza2026-virus-profilers
comparison title: Virus profilers on a shared Virus Pathogen Database (Tisza et al. 2026, developer benchmark)
headline metric: false-positive-count
stratum label: Pathogen-free negative controls
stratum order: 2
review: method: source-hash-verification; independent-cell-check; scope-review; reviewer: claude; reviewer note: Separate Claude review agent (reviewer-path), independent of the extractor; no human review claimed; date: 2026-10-11; artifact sha256: fd92099655ddefdec31d35e273b80c4b233eed395c1cfc137eaddaa4efee232b; retrieval url: https://www.biorxiv.org/content/early/2026/09/30/2026.09.29.755457.source.xml; method note: Judgement drafted by the extracting agent from the pinned preprint and workbook.; note: Re-downloaded the pinned artifact and matched its SHA-256; compared every value with a separate stdlib parser written for this review (the extractor's script was not run) and checked a stratified sample by hand against the rendered table.
reviewed evaluations: rna-pathogen-20261011-tisza2026-eval-neg-centrifuger; rna-pathogen-20261011-tisza2026-eval-neg-esviritu; rna-pathogen-20261011-tisza2026-eval-neg-kraken2; rna-pathogen-20261011-tisza2026-eval-neg-metabuli; rna-pathogen-20261011-tisza2026-eval-neg-sylph
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subject: Select an RNA pathogen-detection workflow for diagnostic testing

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