rewirebio.iobenchmarks
Protocol

Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

Memory needed to store each tool's database or model weights, and reads classified per second with four CPUs.

6 evaluations · 13 results

Overview

An explanatory profile has not yet been reviewed for this record.

Consult the linked sources for architecture or protocol details. Missing evidence is not evidence of a missing capability.

6 recorded evaluations, 13 metric rows. A comparison chart has not yet been validated for these results. The table retains the individual findings and their sources.

View coverage and remaining gaps across all benchmarks

Results

Results are available, but no reviewed comparison panel is linked in this release.

All evaluations

6 evaluations · 13 results. Different protocols are not a single leaderboard.

Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024) · Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)

Sorted by Inference throughput (reads classified per second with four CPUs) (higher is better). The best value in each column is highlighted. Decimals are rounded for display; each value links to the printed value and its source.

Tested configurationInference throughput (reads classified per second with four CPUs)Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)Inference throughput (reads classified per second with four CPUs, including database loading time; value printed in the text)
MetageNN (main database, Peres da Silva et al. 2024)Evaluation1424 (best in column)0.839Not reported
MEGAN-LR (main database, Peres da Silva et al. 2024)Evaluation6065.1Not reported
MMseqs2 (main database, Peres da Silva et al. 2024)Evaluation58827.8Not reported
MetaMaps (main database, Peres da Silva et al. 2024)Evaluation1942.2Not reported
GeNet (main database, Peres da Silva et al. 2024)Evaluation1340.758 (best in column)Not reported
Kraken2 (main database, Peres da Silva et al. 2024)Evaluation13.53.713,471
All 13 result rows with coverage, uncertainty and sources
Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: GeNet (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
134 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

GeNet on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "GeNet", column "Speed (reads/sec)"
Configuration: GeNet (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
0.758 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

GeNet on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "GeNet", column "Memory (GB)"
Configuration: Kraken2 (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
13.5 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Kraken2 on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "Kraken2", column "Speed (reads/sec)"
Configuration: Kraken2 (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
13,471 Inference throughput (reads classified per second with four CPUs, including database loading time; value printed in the text)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Kraken2 on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Results 'MetageNN is faster and requires less storage than alignment and k-mer-based tools, respectively' paragraph 3, sentence 5
Configuration: Kraken2 (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
3.7 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

Kraken2 on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "Kraken2", column "Memory (GB)"
Configuration: MEGAN-LR (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
606 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MEGAN-LR on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MEGAN-LR", column "Speed (reads/sec)"
Configuration: MEGAN-LR (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
5.1 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MEGAN-LR on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MEGAN-LR", column "Memory (GB)"
Configuration: MetageNN (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
1420 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MetageNN on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MetageNN", column "Speed (reads/sec)"
Configuration: MetageNN (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
0.839 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MetageNN on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MetageNN", column "Memory (GB)"
Configuration: MetaMaps (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
194 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MetaMaps on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MetaMaps", column "Speed (reads/sec)"
Configuration: MetaMaps (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
2.2 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MetaMaps on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MetaMaps", column "Memory (GB)"
Configuration: MMseqs2 (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
588 Inference throughput (reads classified per second with four CPUs)
sample-per-second · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MMseqs2 on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MMseqs2", column "Speed (reads/sec)"
Configuration: MMseqs2 (main database, Peres da Silva et al. 2024)Protocol: Database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)
Dataset: ONT reads from 81 bacterial isolates (Peres da Silva et al. 2024)
27.8 Reference storage size (database or index (model weights for MetageNN and GeNet) built from the 516-genome main database)
gigabyte · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

MMseqs2 on database storage and classification speed on ONT data, main database (Peres da Silva et al. 2024)

dna-pathogen-20261012-metagenn-protocol-resources

Aggregation: Not reported

MetageNN: a memory-efficient neural network taxonomic classifier robust to sequencing errors and missing genomes · Table 1, row "MMseqs2", column "Memory (GB)"

Source checking is not independent reproduction. Release 2026-10-22-1b7cf027e4f6.

Methods and evaluation design

Procedure, tasks and evaluated configurations

Recorded evaluations

Each evaluation records what was tested and under which conditions.

Baseline coverage

Reference methods help show what a model adds beyond simple controls. We track a null control and a conventional method for each protocol.

0 of 2 active baseline roles have published Rewire measurements in this release. Measurements on a selected protocol do not establish coverage of an entire suite.

No execution recipe linked to this protocol. Recipe availability does not establish a completed evaluation.

Author-reported evaluations
6

Literature evidence is not a Rewire measurement. Executed but unpublished runs and private review status are not included.

Null control

Proposed control: requires review

Training-set class prior where supervised fitting is permitted

Protocol-specific applicability, permitted inputs, access, split, evaluator and execution requirements need review before implementation or execution.

This is a suggested selection rule, not a validated method or a measured score.

Conventional reference

Proposed control: requires review

Regularised classifier on simple permitted features, or protocol's conventional reference

Protocol-specific applicability, permitted inputs, access, split, evaluator and execution requirements need review before implementation or execution.

This is a suggested selection rule, not a validated method or a measured score.

Protocol coverage CSV (gzip) · Model evaluation matrix (gzip) · Source table (gzip) · Release and checksums (gzip)

Coverage is derived from release 2026-10-22-1b7cf027e4f6. Source citations describe the original records; they do not validate an unreviewed baseline proposal. No results have been generated by this audit.

Run instructions

No runnable recipe has been reviewed for this protocol. Dataset access, model requirements, licences and compute requirements must be checked against its sources before execution.

Strengths, limitations and unresolved questions

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

0 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance

No evidence rows match these filters. Choose another scope or clear the search.

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261012-metagenn-protocol-resources

areas
microbes-communities
contexts
research
protocol
Memory needed to store each tool's database or model weights, and reads classified per second with four CPUs.
version
BMC Bioinformatics 2024
metric definition
Speed includes database loading (Kraken2) and k-mer counting and dataset loading (MetageNN); four CPUs for all tools.
source locator
Table 1; Results 'MetageNN is faster and requires less storage than alignment and k-mer-based tools, respectively'
limitations
The article does not say which ONT reads were timed; the dataset link is the isolate set.
review
method: source-hash-verification; scope-review; reviewer: claude; reviewer note: Separate Claude review agent (rev-mlvb), independent of the extracting agent; no human review claimed; date: 2026-10-12; artifact sha256: ac4b137508d295d5e04e9be263bef40bdcf2c494e096407e5896611194f14e12; retrieval url: https://www.ebi.ac.uk/europepmc/webservices/rest/PMC11022314/fullTextXML; note: Checked against the re-downloaded article XML (SHA-256 matches).
Related records

Suggest a correction