Oxford nasopharyngeal RNA-virus clinical metagenomics workflow (Sanderson et al. 2026)
Host depletion by low-speed centrifugation and M-SAN HQ nuclease, virus precipitation, Direct-zol RNA extraction, cDNA synthesis and SISPA, ONT Rapid Barcoding on R10.4.1 (GridION), and minimap2 mapping to a curated database of the PCR panel's pathogen genomes, with batch and internal controls.
Results are recorded on linked configurations and versions of this method. Their settings and evaluations are kept separate below.
Results on linked records
This method has no results of its own in this release. 2 linked records have results. They are not assigned to the underlying method: each record keeps its own settings and evaluations, and its scores are on its own page.
- Alternative post-hoc detection criterion (Sanderson et al. 2026) Configuration · configuration · 1 evaluation · 6 results
- Pre-specified detection criteria (Sanderson et al. 2026) Configuration · configuration · 1 evaluation · 8 results
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Sources and history
Release 2026-10-22-1b7cf027e4f6 · Record review: source checked
1 source record and release history
- Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens: diagnostic accuracy study · Original source · medRxiv 10.64898/2026.02.06.26345651 v2, posted 2026-02-26; JATS XML
Technical metadata and extraction receipts
Stable ID: rna-pathogen-20261011-sanderson2026-method-cmg-ont
- areas
- microbes-communities
- contexts
- clinical_research
- method types
- conventional_pipeline
- reported name
- CMg workflow
- entity level
- method
- source locator
- Methods 'Procedures' paragraphs 2-4; Figure 1
- access
- Laboratory workflow; code availability is stated in the preprint's 'Code Availability' section (not inspected further)
- missing metadata
- version: reason: inapplicable; note: Family record; criteria are on configurations
Related records
- configuration of: Alternative post-hoc detection criterion (Sanderson et al. 2026)
- configuration of: Pre-specified detection criteria (Sanderson et al. 2026)