Method

BLAST (BLASTn)

Evidence concern: excluded from comparisons

Table 1 prints Kraken relative abundances of 5.17E‐10 (Salmonella enterica and Escherichia coli), 5.17E‐11 (Lactobacillus fermentum) and 5.17E‐12 (Enterococcus faecalis): one mantissa over three orders of magnitude. As read fractions these need at least about 2 billion to 190 billion reads per assigned read, far above the study's libraries (patient samples average 33.5 M reads after QC; the control library size is not given). The values are probably a computation or formatting artefact, so the Kraken column of Table 1 and its eta squared (3.20E‐2) should not be compared. Values are kept as printed. Table 2 values are not affected by this finding.Diagnostic Accuracy of Shotgun Metagenomics for Bloodstream Infections Is Influenced by Bioinformatics Workflow Selection · Table 1, column 'Kraken', rows 'Salmonella enterica' to 'Enterococcus faecalis' and 'η 2'; Methods 'Sample Collection and Processing'

Heuristic nucleotide alignment to the NCBI nt database.

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Heuristic nucleotide alignment to the NCBI nt database.

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Claims, original sources and review scope · Release 2026-10-09-8cc1db47c7f9
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Release 2026-10-09-8cc1db47c7f9 · Record review: source checked

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Diagnostic Accuracy of Shotgun Metagenomics for Bloodstream Infections Is Influenced by Bioinformatics Workflow Selection · Original source · MicrobiologyOpen 14(6):e70158, published online 2025-12-15; PMC12705909 full-text XML

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Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261009-method-blast

areas: microbes-communities
contexts: clinical_research
method types: conventional_pipeline
reported name: BLAST (BLASTn)
entity level: method
source locator: Methods 'Data Analysis' paragraph 1; Discussion paragraph 3
missing metadata: version: reason: inapplicable; note: Family record; versions are on configurations

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configuration of: BLAST (Song et al. 2025)

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