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Evidence claim

Relevance of structural-20261009-protocol-smorodina2026-vhh-iptm-dockq-calibration to "Which predicted interfaces or structures are reliable enough to guide my next experiment?"

Use case
Choose structural hypotheses to guide experiments
Assessed by
How well ipTM tracks DockQ on cognate nanobody-antigen complexes
Relevance
Proxy evidence
Endpoint
Pearson correlation of ipTM with DockQ, share of confident failures and unconfident successes, and correlation of ipTM change with DockQ change under sampling, for AlphaFold3, Boltz-2 and Chai-1 on 106 nanobody-antigen complexes

Measures whether each tool's confidence tracks its structural accuracy on nanobody complexes, which decides whether a confident model can be trusted. Proxy evidence: cognate complexes only, mixed training exposure, preprint.

3 reviewed evaluations

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-11-02-a7f03c9e55a5
Property and statementOriginal source and locationReview and provenance
Citation locations
1 values
  • source id
    structural-20261009-source-smorodina2026
    locator
    Results P18 to P22; Figure 3
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.citation_locators

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Comparison group
nanobody-antigen-interfaces
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.comparison_group

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Comparison title
Nanobody-antigen interface predictions (preprint)
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.comparison_title

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Constraints
4 values
  • Inspect every linked evaluation's source locator and preserved limitations before citing a result.
  • Do not combine this mapping's evaluations with any other protocol's results; complex sets, sampling and metrics differ.
  • Results for one complex class do not establish performance in another.
  • Structural accuracy and confidence are not evidence that an interaction exists, how strong it is or that an experiment will work.
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.constraints

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Endpoint
Pearson correlation of ipTM with DockQ, share of confident failures and unconfident successes, and correlation of ipTM change with DockQ change under sampling, for AlphaFold3, Boltz-2 and Chai-1 on 106 nanobody-antigen complexes
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.endpoint

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Claimed field
links:assessed_by:structural-20261009-protocol-smorodina2026-vhh-iptm-dockq-calibration
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.field

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Headline metric
pearson-correlation
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.headline_metric

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Limitations
9 values
  • Calibration is computed on cognate complexes only; it says nothing about whether ipTM separates binders from non-binders.
  • Quadrant thresholds (DockQ 0.23, ipTM 0.5) are the source's choice, and the text does not say which sample (best, first or worst) the quadrant shares refer to.
  • Not every tool has every value printed in the text.
  • Nanobody (VHH)-antigen complexes only; results do not transfer to conventional antibodies or other complex classes.
  • The set mixes systems inside and outside each tool's training data (AF3 30 of 106 in training, Chai-1 25, Boltz-2 64); the printed values are over all systems, so they are not post-cutoff results, and Boltz-2's are the most exposed.
  • Preprint, not peer reviewed. Supplementary tables were not read.
  • No uncertainty is printed for these values.
  • The dataset paragraph (P62) says post-October 2021 depositions were kept, 'corresponding to the earliest training cutoff among the evaluated tools (Boltz-2)'. The methods (P76) give Chai-1 about 12 January 2021, AF3 about 30 September 2021 and Boltz-2 about 1 June 2023, so Chai-1 is the earliest and Boltz-2 the latest, and the October 2021 date matches AF3. The per-tool train and test counts follow the methods, and no printed value depends on the mis-stated sentence.
  • Preprint, not peer reviewed.
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.limitations

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Pinned records
18 values
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Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.pins

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

Rationale
Measures whether each tool's confidence tracks its structural accuracy on nanobody complexes, which decides whether a confident model can be trusted. Proxy evidence: cognate complexes only, mixed training exposure, not yet peer reviewed.
Context-only references		Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence Scores

Original source ↗

structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3

Version: bioRxiv version 1, posted 2026-03-03; Europe PMC preprint full text PPR1221387 (manuscript EMS215481); not peer reviewed
Retrieved: 2026-10-09T21:25:48Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.rationale

Source artifact SHA-256: 0ad24054d98fd1888fa8bf3120f76e22f7bf6b1965261b16f045986d72c50732

Hash scope: JATS XML parse (xml.etree), by extract/extract_structural.py

Inspected artifact

How to read the evidence

Sources and history

Release 2026-11-02-a7f03c9e55a5 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: use-case-mapping-structural-20261009-smorodina2026-calibration

field
links:assessed_by:structural-20261009-protocol-smorodina2026-vhh-iptm-dockq-calibration
value
structural-20261009-protocol-smorodina2026-vhh-iptm-dockq-calibration
relevance
proxy
endpoint
Pearson correlation of ipTM with DockQ, share of confident failures and unconfident successes, and correlation of ipTM change with DockQ change under sampling, for AlphaFold3, Boltz-2 and Chai-1 on 106 nanobody-antigen complexes
rationale
Measures whether each tool's confidence tracks its structural accuracy on nanobody complexes, which decides whether a confident model can be trusted. Proxy evidence: cognate complexes only, mixed training exposure, not yet peer reviewed.
constraints
Inspect every linked evaluation's source locator and preserved limitations before citing a result.; Do not combine this mapping's evaluations with any other protocol's results; complex sets, sampling and metrics differ.; Results for one complex class do not establish performance in another.; Structural accuracy and confidence are not evidence that an interaction exists, how strong it is or that an experiment will work.
limitations
Calibration is computed on cognate complexes only; it says nothing about whether ipTM separates binders from non-binders.; Quadrant thresholds (DockQ 0.23, ipTM 0.5) are the source's choice, and the text does not say which sample (best, first or worst) the quadrant shares refer to.; Not every tool has every value printed in the text.; Nanobody (VHH)-antigen complexes only; results do not transfer to conventional antibodies or other complex classes.; The set mixes systems inside and outside each tool's training data (AF3 30 of 106 in training, Chai-1 25, Boltz-2 64); the printed values are over all systems, so they are not post-cutoff results, and Boltz-2's are the most exposed.; Preprint, not peer reviewed. Supplementary tables were not read.; No uncertainty is printed for these values.; The dataset paragraph (P62) says post-October 2021 depositions were kept, 'corresponding to the earliest training cutoff among the evaluated tools (Boltz-2)'. The methods (P76) give Chai-1 about 12 January 2021, AF3 about 30 September 2021 and Boltz-2 about 1 June 2023, so Chai-1 is the earliest and Boltz-2 the latest, and the October 2021 date matches AF3. The per-tool train and test counts follow the methods, and no printed value depends on the mis-stated sentence.; Preprint, not peer reviewed.
citation locators
source id: structural-20261009-source-smorodina2026; locator: Results P18 to P22; Figure 3
source locator
structural-20261009-source-smorodina2026: Results P18 to P22; Figure 3
revision
1
reason
Add comparisons of structure-prediction reliability by complex class from the structural hypotheses use-case pass 2026-10-09.
comparison group
nanobody-antigen-interfaces
comparison title
Nanobody-antigen interface predictions (preprint)
headline metric
pearson-correlation
stratum label
Confidence against accuracy
stratum order
2
reviewed evaluations
structural-20261009-eval-smorodina2026-vhh-iptm-dockq-calibration-af3; structural-20261009-eval-smorodina2026-vhh-iptm-dockq-calibration-boltz2; structural-20261009-eval-smorodina2026-vhh-iptm-dockq-calibration-chai1
review
method: automated-source-review; reviewer: claude; reviewer note: Separate Claude review agent, independent of the extractor; no human review claimed; reviewed at: 2026-10-10T00:30:00Z; note: Independent review of endpoint fit, relevance grade, limitations, exclusions and grouping against the use case and the preprint; every value of the listed evaluations was checked against the paragraph its locator names. Preprint; no execution, reproduction, human scientific review or clinical validation.
pins
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