Evidence claim

Relevance of Predictive specificity in control samples (Wolf et al. 2026 Table 3) to "Select a DNA pathogen-identification workflow for diagnostic testing"

Use case: Select a DNA pathogen-identification workflow for diagnostic testing
Assessed by: Predictive specificity in control samples (Wolf et al. 2026 Table 3)
Relevance: Proxy evidence
Endpoint: Predictive specificity in 162 control samples for any bacterium or yeast and for common bloodstream infection pathogens, with all positive tests and with a post-hoc 140 molecules per microlitre cut-off

Detections in control plasma from patients without fever or infection measure background and clinically false-positive signal, which bears on the use case's contamination question. Proxy evidence: afebrile controls rather than patients tested for suspected infection.

2 reviewed evaluations

Evidence

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Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Citation locations 1 values source id dna-pathogen-20261011-wolf2026-source locator Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis' Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.citation_locators` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison group wolf2026-plasma-mcfdna Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_group` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison title Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026) Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_title` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Constraints 2 values Inspect every linked evaluation's source locator and preserved limitations before citing a result. Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources. Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.constraints` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Endpoint Predictive specificity in 162 control samples for any bacterium or yeast and for common bloodstream infection pathogens, with all positive tests and with a post-hoc 140 molecules per microlitre cut-off Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.endpoint` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Claimed field links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-specificity Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.field` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Headline metric specificity Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.headline_metric` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Limitations 6 values Single commercial assay (Karius); no comparator workflow on the same samples. Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius. Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population. Reference for sensitivity is blood culture. The 140 molecules per microlitre cut-off is post hoc (Youden index on the same data). Burkholderia was excluded after the fact because of possible local sample contamination (Methods 'Statistical analysis'). Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.limitations` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Pinned records 13 values record id dna-pathogen-20261011-wolf2026-eval-specificity-all fields status links attributes.origin attributes.comparison sha256 ce606e24dac9303a9ff44b4e1ad7fa66fe7a6326e89c4208aa07a5286f047bd8 record id dna-pathogen-20261011-wolf2026-eval-specificity-cutoff140 fields status links attributes.origin attributes.comparison sha256 a404b890699dda8198b048c31f175e15222c18b8ac5f821369bec2560700d9c0 record id dna-pathogen-20261011-wolf2026-protocol-specificity fields status name description facets source_ids links attributes sha256 437ef837d49f5c7bfc48d44db92f7d9a7d7db2b748eefb35895af7efaf400b78 record id dna-pathogen-20261011-wolf2026-result-specificity-any-all-negative fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 aa7b290e4b826e4b5a6f91297894b32364ab7e1c662ee97c3ca1f936d768c699 record id dna-pathogen-20261011-wolf2026-result-specificity-any-all-specificity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 d75a52fabb0329eb507c4fa1f762c14adc7ae8ac53905848622fe1dcb4a79a9c record id dna-pathogen-20261011-wolf2026-result-specificity-any-cutoff140-negative fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 b1012b729a4ca716039221e61e95dc02c300077a095f2bd4d8e8b31b3082f584 record id dna-pathogen-20261011-wolf2026-result-specificity-any-cutoff140-specificity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 0a8c3a1aecfff7689ff3ab2743ef0c463c057f3a13a47bdfa044327fb535e940 record id dna-pathogen-20261011-wolf2026-result-specificity-common-all-negative fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 3faa722205ee292eb095c42d6fb3f33f9cb721858af1006eec773adfced650e6 record id dna-pathogen-20261011-wolf2026-result-specificity-common-all-specificity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 6892d97c946d9d94a7e616585f8e0a63f35d6cc670860e84c5176339f1daa786 record id dna-pathogen-20261011-wolf2026-result-specificity-common-cutoff140-negative fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 802310bed40e5f007d3bcf1e48957214acd3bc59fafbca64cca1c2027bc5b4e1 record id dna-pathogen-20261011-wolf2026-result-specificity-common-cutoff140-specificity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 54aa8189c76c3c3b5581d7870971b830272a07b54042734fb68fb274bef478ce record id dna-pathogen-20261011-wolf2026-source fields status attributes.artifact_sha256 sha256 9b0346b6b44bf87369b1b81ba49bea4c7319207c7c4a87308233692ee311dec7 record id use-case-diagnostic-dna-pathogen-identification fields status name description facets attributes.question attributes.decision attributes.inputs attributes.output attributes.exclusions attributes.setting attributes.clinical_scope attributes.intended_users sha256 fc133441f475bb23dafd0c0c55f4e16cd20302f9148b21b98de31077dfe16338 Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.pins` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Rationale Detections in control plasma from patients without fever or infection measure background and clinically false-positive signal, which bears on the use case's contamination question. Proxy evidence: afebrile controls rather than patients tested for suspected infection. Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 3 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.rationale` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

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Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

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Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Original source · The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)

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Technical metadata and extraction receipts

Stable ID: use-case-mapping-dna-pathogen-20261011-wolf2026-specificity

field: links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-specificity
value: dna-pathogen-20261011-wolf2026-protocol-specificity
relevance: proxy
endpoint: Predictive specificity in 162 control samples for any bacterium or yeast and for common bloodstream infection pathogens, with all positive tests and with a post-hoc 140 molecules per microlitre cut-off
rationale: Detections in control plasma from patients without fever or infection measure background and clinically false-positive signal, which bears on the use case's contamination question. Proxy evidence: afebrile controls rather than patients tested for suspected infection.
constraints: Inspect every linked evaluation's source locator and preserved limitations before citing a result.; Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources.
limitations: Single commercial assay (Karius); no comparator workflow on the same samples.; Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius.; Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population.; Reference for sensitivity is blood culture.; The 140 molecules per microlitre cut-off is post hoc (Youden index on the same data).; Burkholderia was excluded after the fact because of possible local sample contamination (Methods 'Statistical analysis').
citation locators: source id: dna-pathogen-20261011-wolf2026-source; locator: Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis'
source locator: dna-pathogen-20261011-wolf2026-source: Table 3
revision: 1
reason: Recorded from the 2026-10-11 DNA pathogen-identification literature pass (data/omics/use-case-coverage-dna-pathogen-20261011-wolf2026). Independently reviewed in docs/reviews/use-cases/pathogen-use-cases-2026-10-11.md.
comparison group: wolf2026-plasma-mcfdna
comparison title: Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026)
headline metric: specificity
stratum label: Control samples
stratum order: 3
review: method: source-hash-verification; independent-cell-check; scope-review; reviewer: claude; reviewer note: Separate Claude review agent (reviewer-path), independent of the extractor; no human review claimed; date: 2026-10-11; artifact sha256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689; retrieval url: https://www.ebi.ac.uk/europepmc/webservices/rest/PMC13154368/fullTextXML; method note: Judgement drafted by the extracting agent from the pinned article.; note: Re-downloaded the pinned artifact and matched its SHA-256; compared every value with a separate stdlib parser written for this review (the extractor's script was not run) and checked a stratified sample by hand against the rendered table.
reviewed evaluations: dna-pathogen-20261011-wolf2026-eval-specificity-all; dna-pathogen-20261011-wolf2026-eval-specificity-cutoff140
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