Evidence claim

Relevance of Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2) to "Select a DNA pathogen-identification workflow for diagnostic testing"

Use case: Select a DNA pathogen-identification workflow for diagnostic testing
Assessed by: Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2)
Relevance: Proxy evidence
Endpoint: Episode-level predictive sensitivity (day -3 to -1) against all blood-culture organisms, raw and with imputation

Detection of the blood-culture organism in plasma cell-free DNA in the three days before onset bears on the assay's detection limits in the use case's specimen, but prediction before clinical presentation is a different decision from diagnostic testing. Proxy evidence.

1 reviewed evaluation

Karius mcfDNA-Seq (all positive tests) on Predictive sensitivity, day -3 to -1 (Wolf et al. 2026 Table 2)

Evidence

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Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statement	Original source and location	Review and provenance
Citation locations 1 values source id dna-pathogen-20261011-wolf2026-source locator Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis' Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.citation_locators` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison group wolf2026-plasma-mcfdna Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_group` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Comparison title Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026) Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.comparison_title` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Constraints 2 values Inspect every linked evaluation's source locator and preserved limitations before citing a result. Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources. Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.constraints` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Endpoint Episode-level predictive sensitivity (day -3 to -1) against all blood-culture organisms, raw and with imputation Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.endpoint` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Claimed field links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-prediction Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.field` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Headline metric recall Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.headline_metric` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Limitations 4 values Single commercial assay (Karius); no comparator workflow on the same samples. Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius. Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population. Reference for sensitivity is blood culture. Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.limitations` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Pinned records 22 values record id dna-pathogen-20261011-wolf2026-eval-prediction fields status links attributes.origin attributes.comparison sha256 d68100a5d0c16f1301a96d0ab6d2727a2c6f0d1d031cd2da56ede704b1c27bd5 record id dna-pathogen-20261011-wolf2026-protocol-prediction fields status name description facets source_ids links attributes sha256 c71f4829eddfaac134ab880d12a20ba193820c2a375f4a8dd44bc1185403e0e9 record id dna-pathogen-20261011-wolf2026-result-prediction-all-imputed-evaluable fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 1a667dd1715f598c18cfdb0637c457fc21ed6db4f109396ba71e755d049e9d08 record id dna-pathogen-20261011-wolf2026-result-prediction-all-imputed-positive fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 03ffc0738268571da7cbdd3fe12017ba17fc7c645f780d1dd5d483f25abec4bd record id dna-pathogen-20261011-wolf2026-result-prediction-all-imputed-sensitivity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 ebb43cccea181ae19fb9b08d98882d8f9a08e13a69d587524aa6c021c65053f7 record id dna-pathogen-20261011-wolf2026-result-prediction-all-raw-evaluable fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 467f81852f3490ae1d34eaafa060209ca162f97d0d178f611094c129c8a7a12e record id dna-pathogen-20261011-wolf2026-result-prediction-all-raw-positive fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 f1460e487eea0458dfbda13fb3a7bb57225638a94c922afe9d302a799be719d9 record id dna-pathogen-20261011-wolf2026-result-prediction-all-raw-sensitivity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 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attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 352acf9d8a8c95558c72e6371119059d31dbfb9d6df3e9eab3a7e401f91eb8b9 record id dna-pathogen-20261011-wolf2026-result-prediction-bacterial-raw-positive fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 b3e91d987ab190d42712a8058e97dee3aea8df91cb0708da050698cd0b14fade record id dna-pathogen-20261011-wolf2026-result-prediction-bacterial-raw-sensitivity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 8e321f39b29e7e019e5d61b31e14ae66c694a366506c6d3bf5eaa122721b90a3 record id dna-pathogen-20261011-wolf2026-result-prediction-common-imputed-evaluable fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 2ca92a96e8fe0d33a537d8ebcf22be96615cf7161dc5b67d3d71b1ff2becd887 record id dna-pathogen-20261011-wolf2026-result-prediction-common-imputed-positive fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 7350192353dd5ef48807049e19d404110e84561e19df8461359d46ade7397f55 record id dna-pathogen-20261011-wolf2026-result-prediction-common-imputed-sensitivity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 85d0f240c8390b08fad6ba6e19afeae5c0ff53efb8aa106e04d3feb5130b4e6a record id dna-pathogen-20261011-wolf2026-result-prediction-common-raw-evaluable fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 127b84f045fbc1c9b884cb419442f765bfa9c70f01400538b3206a6a7e2929c0 record id dna-pathogen-20261011-wolf2026-result-prediction-common-raw-positive fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 3b7fbf6f72a0cf4cd5bbd69159ed583d2e2f99a6a5a1e74a84f4dc30c5e32e87 record id dna-pathogen-20261011-wolf2026-result-prediction-common-raw-sensitivity fields status attributes.metric attributes.metric_qualifier attributes.printed_value attributes.numeric_value sha256 193d20d257568bfea62ff8dc22b40588434c1c80045e7c85cd977584034f800b record id dna-pathogen-20261011-wolf2026-source fields status attributes.artifact_sha256 sha256 9b0346b6b44bf87369b1b81ba49bea4c7319207c7c4a87308233692ee311dec7 record id use-case-diagnostic-dna-pathogen-identification fields status name description facets attributes.question attributes.decision attributes.inputs attributes.output attributes.exclusions attributes.setting attributes.clinical_scope attributes.intended_users sha256 fc133441f475bb23dafd0c0c55f4e16cd20302f9148b21b98de31077dfe16338 Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.pins` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact
Rationale Detection of the blood-culture organism in plasma cell-free DNA in the three days before onset bears on the assay's detection limits in the use case's specimen, but prediction before clinical presentation is a different decision from diagnostic testing. Proxy evidence. Context-only references	Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study Original source ↗ dna-pathogen-20261011-wolf2026-source: Table 2 Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript) Retrieved: 2026-10-11T22:07:34Z	not individually reviewed No individual claim review recorded Audit details Field: `attributes.rationale` Source artifact SHA-256: `0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689` Hash scope: Hash scope not separately documented; inspect source record Inspected artifact

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Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release history

Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study · Original source · The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)

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Technical metadata and extraction receipts

Stable ID: use-case-mapping-dna-pathogen-20261011-wolf2026-prediction

field: links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-prediction
value: dna-pathogen-20261011-wolf2026-protocol-prediction
relevance: proxy
endpoint: Episode-level predictive sensitivity (day -3 to -1) against all blood-culture organisms, raw and with imputation
rationale: Detection of the blood-culture organism in plasma cell-free DNA in the three days before onset bears on the assay's detection limits in the use case's specimen, but prediction before clinical presentation is a different decision from diagnostic testing. Proxy evidence.
constraints: Inspect every linked evaluation's source locator and preserved limitations before citing a result.; Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources.
limitations: Single commercial assay (Karius); no comparator workflow on the same samples.; Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius.; Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population.; Reference for sensitivity is blood culture.
citation locators: source id: dna-pathogen-20261011-wolf2026-source; locator: Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis'
source locator: dna-pathogen-20261011-wolf2026-source: Table 2
revision: 1
reason: Recorded from the 2026-10-11 DNA pathogen-identification literature pass (data/omics/use-case-coverage-dna-pathogen-20261011-wolf2026). Independently reviewed in docs/reviews/use-cases/pathogen-use-cases-2026-10-11.md.
comparison group: wolf2026-plasma-mcfdna
comparison title: Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026)
headline metric: recall
stratum label: Prediction (day -3 to -1)
stratum order: 2
review: method: source-hash-verification; independent-cell-check; scope-review; reviewer: claude; reviewer note: Separate Claude review agent (reviewer-path), independent of the extractor; no human review claimed; date: 2026-10-11; artifact sha256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689; retrieval url: https://www.ebi.ac.uk/europepmc/webservices/rest/PMC13154368/fullTextXML; method note: Judgement drafted by the extracting agent from the pinned article.; note: Re-downloaded the pinned artifact and matched its SHA-256; compared every value with a separate stdlib parser written for this review (the extractor's script was not run) and checked a stratified sample by hand against the rendered table.
reviewed evaluations: dna-pathogen-20261011-wolf2026-eval-prediction
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subject: Select a DNA pathogen-identification workflow for diagnostic testing

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