rewirebio.iobenchmarks
Evidence claim

Relevance of Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2) to "Select a DNA pathogen-identification workflow for diagnostic testing"

Use case
Select a DNA pathogen-identification workflow for diagnostic testing
Assessed by
Diagnostic sensitivity, day 0 or 1 (Wolf et al. 2026 Table 2)
Relevance
Proxy evidence
Endpoint
Episode-level diagnostic sensitivity (day 0 or 1) against all blood-culture organisms, raw and with imputation, overall, bacterial and common pathogens

Diagnostic sensitivity of the Karius plasma cell-free DNA assay against blood-culture organisms at bloodstream infection onset matches the use case's inputs and endpoint (agreement with blood culture for plasma microbial cell-free DNA), measured prospectively in a second population. It is proxy evidence for the same reason as the Karius anchor: blood culture does not identify every infection, so this is agreement with culture, not sensitivity against all infections. The population is children and young adults with high-risk leukaemia, and Karius took part in processing and analysis.

1 reviewed evaluation

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Citation locations
1 values
  • source id
    dna-pathogen-20261011-wolf2026-source
    locator
    Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis'
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.citation_locators

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison group
wolf2026-plasma-mcfdna
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.comparison_group

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Comparison title
Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026)
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.comparison_title

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Constraints
2 values
  • Inspect every linked evaluation's source locator and preserved limitations before citing a result.
  • Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources.
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.constraints

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Endpoint
Episode-level diagnostic sensitivity (day 0 or 1) against all blood-culture organisms, raw and with imputation, overall, bacterial and common pathogens
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.endpoint

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Claimed field
links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-diagnosis
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.field

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Headline metric
recall
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.headline_metric

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Limitations
4 values
  • Single commercial assay (Karius); no comparator workflow on the same samples.
  • Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius.
  • Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population.
  • Reference for sensitivity is blood culture.
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.limitations

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Pinned records
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Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.pins

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Rationale
Diagnostic sensitivity of the Karius plasma cell-free DNA assay against blood-culture organisms at bloodstream infection onset matches the use case's inputs and endpoint (agreement with blood culture for plasma microbial cell-free DNA), measured prospectively in a second population. It is proxy evidence for the same reason as the Karius anchor: blood culture does not identify every infection, so this is agreement with culture, not sensitivity against all infections. The population is children and young adults with high-risk leukaemia, and Karius took part in processing and analysis.
Context-only references
Predicting bloodstream infection by plasma cell-free metagenomic sequencing: a prospective cohort study

Original source ↗

dna-pathogen-20261011-wolf2026-source: Table 2

Version: The Lancet Microbe 7:101312, published online 2026-03-02; PMC13154368 full-text XML (author manuscript)
Retrieved: 2026-10-11T22:07:34Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.rationale

Source artifact SHA-256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: use-case-mapping-dna-pathogen-20261011-wolf2026-diagnosis

field
links:assessed_by:dna-pathogen-20261011-wolf2026-protocol-diagnosis
value
dna-pathogen-20261011-wolf2026-protocol-diagnosis
relevance
proxy
endpoint
Episode-level diagnostic sensitivity (day 0 or 1) against all blood-culture organisms, raw and with imputation, overall, bacterial and common pathogens
rationale
Diagnostic sensitivity of the Karius plasma cell-free DNA assay against blood-culture organisms at bloodstream infection onset matches the use case's inputs and endpoint (agreement with blood culture for plasma microbial cell-free DNA), measured prospectively in a second population. It is proxy evidence for the same reason as the Karius anchor: blood culture does not identify every infection, so this is agreement with culture, not sensitivity against all infections. The population is children and young adults with high-risk leukaemia, and Karius took part in processing and analysis.
constraints
Inspect every linked evaluation's source locator and preserved limitations before citing a result.; Do not combine these results with another protocol's results; reference standards, specimens and scoring units differ between sources.
limitations
Single commercial assay (Karius); no comparator workflow on the same samples.; Karius provided testing and took part in sample processing and analysis, data management and manuscript review; one author (AAA) is a Karius employee and coinventor on a pending Karius patent, and two others report research or sequencing support from Karius.; Paediatric and young-adult high-risk leukaemia population; not a general sepsis-alert population.; Reference for sensitivity is blood culture.
citation locators
source id: dna-pathogen-20261011-wolf2026-source; locator: Table 2; Table 3; Methods 'Outcomes' and 'Statistical analysis'
source locator
dna-pathogen-20261011-wolf2026-source: Table 2
revision
1
reason
Recorded from the 2026-10-11 DNA pathogen-identification literature pass (data/omics/use-case-coverage-dna-pathogen-20261011-wolf2026). Independently reviewed in docs/reviews/use-cases/pathogen-use-cases-2026-10-11.md.
comparison group
wolf2026-plasma-mcfdna
comparison title
Karius plasma mcfDNA sequencing in high-risk paediatric leukaemia (Wolf et al. 2026)
headline metric
recall
stratum label
Diagnosis (day 0 or 1)
stratum order
1
review
method: source-hash-verification; independent-cell-check; scope-review; reviewer: claude; reviewer note: Separate Claude review agent (reviewer-path), independent of the extractor; no human review claimed; date: 2026-10-11; artifact sha256: 0f6905d0a0c9ace686bcc61970c711558ccf530b5c31f882607a305f8b5ca689; retrieval url: https://www.ebi.ac.uk/europepmc/webservices/rest/PMC13154368/fullTextXML; method note: Judgement drafted by the extracting agent from the pinned article.; note: Re-downloaded the pinned artifact and matched its SHA-256; compared every value with a separate stdlib parser written for this review (the extractor's script was not run) and checked a stratified sample by hand against the rendered table.
reviewed evaluations
dna-pathogen-20261011-wolf2026-eval-diagnosis
pins
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