rewire.itbenchmarks
Task

Strain-level abundance quantification

Strain-level taxonomic abundance estimation is compared against known compositions in simulated human-gut read collections.

SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

2 evaluations · 2 results

Overview

Datasets

Queries derived from a reference index of human-gut bacterial genomes; Table 5 reports four strain-level query sets.

Metrics

Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.

Allowed inputs

Metagenomic reads and a reference genome index.

SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
Evaluation procedure diagram
How it worksComputational evaluation flow
Computational evaluation flow1. Input: Metagenomic reads and a reference genome index.. Then: 2. Evaluation: An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.. Then: 3. Readout: Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.Computational evaluation flow1. Input: Metagenomic reads and a reference genome index.. Then: 2. Evaluation: An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.. Then: 3. Readout: Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.Computational evaluation flow1. Input: Metagenomic reads and a reference genome index.. Then: 2. Evaluation: An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.. Then: 3. Readout: Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.

Conceptual summary of the cited evaluation; exact task configuration and source version remain part of the protocol.

SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

limited source coverage · Automated source review, 2026-09-16. All specifications and missing details

Results

Results are available, but no reviewed comparison panel is linked in this release.

All evaluations

2 evaluations · 2 results. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: CAMMiQTask: Strain-level abundance quantification
Dataset: HumanGut-all strain-level query
0.0517 L1 abundance error
unitless · unknown

Uncertainty: Not reported

Coverage: Not reported scored / Not reported eligible

Author-reported evaluation · Source checked
Methods, coverage and source

CAMMiQ: Strain-level abundance quantification

Strain-level quantification on the HumanGut-all synthetic query.

Aggregation: Not reported

Strain level microbial detection and quantification with applications to single cell metagenomics · Table 5, B. L1 Err. / HumanGut-all row, CAMMiQ L1 Err. column
Configuration: Kraken2Task: Strain-level abundance quantification
Dataset: HumanGut-all strain-level query
0.284 L1 abundance error
unitless · unknown

Uncertainty: Not reported

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2: Strain-level abundance quantification

Strain-level quantification on the HumanGut-all synthetic query.

Aggregation: Not reported

Strain level microbial detection and quantification with applications to single cell metagenomics · Table 5, B. L1 Err. / HumanGut-all row, Kraken2 L1 Err. column

Source checking is not independent reproduction. Release 2026-09-29-06401fd5b220.

Methods and evaluation design

Procedure, tasks and evaluated configurations

How it works

Evaluation methodology

Queries derived from a reference index of human-gut bacterial genomes; Table 5 reports four strain-level query sets. An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection. Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors. Kraken2, KrakenUniq, CLARK, Centrifuge and MetaPhlAn2. The four strain-level queries are simulated from the same 614-genome reference collection used to build the custom indices. A genome without unique or doubly unique 100-mers is excluded from query generation. This is identification and abundance estimation against an indexed collection, not a strain-held-out generalization test.

Sources (2)Strain level microbial detection and quantification with applications to single cell metagenomics; cammiq-2022__41467_2022_33869_MOESM1_ESM.pdf · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57; Supplementary Notes 5.2 Strain-Level Index Dataset and 5.5 Strain-Level Queries

Recorded evaluations

Each evaluation records what was tested and under which conditions.

Run instructions

No runnable recipe has been reviewed for this task. Dataset access, model requirements, licences and compute requirements must be checked against its sources before execution.

A task describes a biological question. Choose a linked protocol to obtain concrete split and scoring instructions.

Strengths, limitations and unresolved questions

Strengths and limitations

Profile review details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Stable record: reported-task-571f0a2e7faed3

Specifications

Inputs, training, access and other details

Explanatory profile: limited source coverage · Automated source review, 2026-09-16. Review applies to the cited claims; unresolved fields are listed below. Numerical results retain their own review status.

Data, procedure and scoring
PropertyDescription and evidence
DatasetsQueries derived from a reference index of human-gut bacterial genomes; Table 5 reports four strain-level query sets.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
SplitsAn index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
MetricsCorrectly identified strains and L1/L2 distances between predicted and true relative abundance vectors.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
BaselinesKraken2, KrakenUniq, CLARK, Centrifuge and MetaPhlAn2.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
Leakage controlsThe four strain-level queries are simulated from the same 614-genome reference collection used to build the custom indices. A genome without unique or doubly unique 100-mers is excluded from query generation. This is identification and abundance estimation against an indexed collection, not a strain-held-out generalization test.
Sources (2)Strain level microbial detection and quantification with applications to single cell metagenomics; cammiq-2022__41467_2022_33869_MOESM1_ESM.pdf · Supplementary Notes 5.2 Strain-Level Index Dataset and 5.5 Strain-Level Queries
UncertaintyTable 5 reports one identification count and L1/L2 abundance error for each of four different query constructions. Supplementary Note 5.5 does not define these four constructions as replicate draws for a confidence interval, and no uncertainty interval accompanies the table values. · Not reported in inspected sources
Sources (2)Strain level microbial detection and quantification with applications to single cell metagenomics; cammiq-2022__41467_2022_33869_MOESM1_ESM.pdf · Table 5; Supplementary Note 5.5 Strain-Level Queries
Entity typePaper-specific computational evaluation protocol.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
OrganismsHuman-gut bacterial strains.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
AssaysSynthetic sequence queries with known strain abundances.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
Allowed inputsMetagenomic reads and a reference genome index.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57
AdaptationReference-index construction and query classification; no assay-label supervised fitting is prescribed.
SourcesStrain level microbial detection and quantification with applications to single cell metagenomics · Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Papers and result coverage

Last literature check: 2026-09-17. Primary-source discovery and table/protocol screening; source checked is not independently reproduced. Raw acquisitions not automatically numerical publication approval.

Paper or primary resourceVersionReference
Strain level microbial detection and quantification with applications to single cell metagenomicsversion of recordRead source
DOI: 10.1038/s41467-022-33869-7
Historical gaps recorded on 2026-09-17

The catalogue now holds 2 result rows for this benchmark. A note below about pending extraction describes the state on 2026-09-17 and may since have been answered by a later batch. The result rows and their sources are the current record.

  • Complete raw table acquired. Identified-strain counts/ratios and L1/L2 abundance errors must remain different metrics; MetaPhlAn detection bounds are not exact counts. Structured extraction pending.
Search and extraction details

source found structured extraction pending

Searches

  • Strain level microbial detection and quantification with applications to single cell metagenomics primary paper benchmark results

Evidence locations

  • Table5, human-gut mixtures and six methods

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

21 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-09-29-06401fd5b220
Property and statementOriginal source and locationReview and provenance
Diagram caption
Conceptual summary of the cited evaluation; exact task configuration and source version remain part of the protocol.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.diagram.caption

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram steps
  • Input: Metagenomic reads and a reference genome index.
  • Evaluation: An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.
  • Readout: Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.diagram.steps

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram title
Computational evaluation flow
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.diagram.title

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Datasets
Queries derived from a reference index of human-gut bacterial genomes; Table 5 reports four strain-level query sets.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.0.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Splits
An index-and-query benchmark rather than a supervised train/test split; the relevant evaluation is the strain-level collection.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.1.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Adaptation
Reference-index construction and query classification; no assay-label supervised fitting is prescribed.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.10.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Metrics
Correctly identified strains and L1/L2 distances between predicted and true relative abundance vectors.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.2.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Baselines
Kraken2, KrakenUniq, CLARK, Centrifuge and MetaPhlAn2.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.3.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Leakage controls
The four strain-level queries are simulated from the same 614-genome reference collection used to build the custom indices. A genome without unique or doubly unique 100-mers is excluded from query generation. This is identification and abundance estimation against an indexed collection, not a strain-held-out generalization test.
Individual claims
Strain level microbial detection and quantification with applications to single cell metagenomics

Original source ↗

Supplementary Notes 5.2 Strain-Level Index Dataset and 5.5 Strain-Level Queries

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: version of record
Retrieved: 2026-09-16T10:44:03.408237+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: f0939647ed3de995d58254f79472a612c21b0e1b2560a82783302aa1a148dde3

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Leakage controls
The four strain-level queries are simulated from the same 614-genome reference collection used to build the custom indices. A genome without unique or doubly unique 100-mers is excluded from query generation. This is identification and abundance estimation against an indexed collection, not a strain-held-out generalization test.
Individual claims
cammiq-2022__41467_2022_33869_MOESM1_ESM.pdf

Original source ↗

Supplementary Notes 5.2 Strain-Level Index Dataset and 5.5 Strain-Level Queries

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Retrieved 2026-09-16; sha256:910aed130f3b4648b0758bdcc6b82d1e2d38ddb320ea80d670c98c565930610b
Retrieved: 2026-09-16T21:08:58.951180+00:00

source checked

automated source review · 2026-09-16

Audit details

Targeted full-paper and supplement review of the outstanding task fields, with original dataset metadata checked where accessible. Source-scoped omissions are explicit; no independent benchmark reproduction or numerical-result change.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: 910aed130f3b4648b0758bdcc6b82d1e2d38ddb320ea80d670c98c565930610b

Hash scope: Hash scope not separately documented; inspect source record

Archive member: 41467_2022_33869_MOESM1_ESM.pdf

Inspected artifact

Sources and history

View linked audit checks and correction history

Release 2026-09-29-06401fd5b220 · Record review: needs review

3 source records and release historyDownload this release
Technical metadata and extraction receipts

Stable ID: reported-task-571f0a2e7faed3

areas
microbes-communities
tasks
Strain-level abundance quantification
entity level
task
version
Not reported
task
Strain-level abundance quantification
scope note
Paper-specific evaluation task; protocol completeness requires further extraction.
benchmark research
review date: 2026-09-17; status: source_found_structured_extraction_pending; primary sources: evidence-expansion-p2-cammiq-2022-f0939647ed3d; inspected locators: Table5, human-gut mixtures and six methods; searched queries: Strain level microbial detection and quantification with applications to single cell metagenomics primary paper benchmark results; gaps: Complete raw table acquired. Identified-strain counts/ratios and L1/L2 abundance errors must remain different metrics; MetaPhlAn detection bounds are not exact counts. Structured extraction pending.; claim scope: Primary-source discovery and table/protocol screening; source checked is not independently reproduced. Raw acquisitions not automatically numerical publication approval.
historical missing metadata
protocol version: not_reported_in_legacy_extract; split: not_reported_in_legacy_extract
metadata review scope
historical_missing_metadata preserves the original discovery state. Current descriptive evidence and missingness are recorded in profile.facts; numerical-result review is separate.
legacy kinds
benchmark
entity classification
review date: 2026-09-17; rationale: This source-scoped record identifies the biological prediction task and holds its paper context. Preserve the existing task identity; exact split, model adaptation and scoring remain in linked evaluations or separate protocol records.; source ids: cammiq-2022; source locator: Results: Datasets; Performance of CAMMiQ at the strain level; Table 5; cached text lines 19–22, 55–57; ambiguities: A paper- or suite-specific task may constrain some inputs or metrics; that alone does not make it interchangeable with a complete versioned protocol. No protocol equivalence is inferred.; Some legacy profile Entity type facts use the generic phrase computational evaluation protocol. That boilerplate is not sufficient to establish a single fixed protocol identity or to merge this task with another protocol record.
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