Fine-tuned RFdiffusion antibody design pipeline (RFantibody)
A version of RFdiffusion fine-tuned predominantly on antibody complex structures designs antibody variable-domain backbones against a specified epitope, ProteinMPNN designs their sequences, and a fine-tuned RoseTTAFold2 filters the designs.
Results are recorded on linked configurations and versions of this pipeline. Their settings and evaluations are kept separate below.
Results on linked records
This pipeline has no results of its own in this release. One linked record has results. They are not assigned to the underlying pipeline: each record keeps its own settings and evaluations, and its scores are on its own page.
- Fine-tuned RFdiffusion VHH design with fine-tuned RoseTTAFold2 filtering at hotspot proportion 1 Configuration · configuration · 30 evaluations · 36 results
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Evidence
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Sources and history
Release 2026-10-22-1b7cf027e4f6 · Record review: source checked
1 source record and release history
- Atomically accurate de novo design of antibodies with RFdiffusion (Bennett et al., Nature 2025) · Original source · Nature, version of record; Europe PMC JATS full text for PMC12727541
Technical metadata and extraction receipts
Stable ID: uc-binder-20261022-pipeline-bennett-rfdiffusion-antibody
- areas
- proteins-complexes; protein-structure
- contexts
- research
- method types
- specialist
- access
- Released as a single repository, free for academic, personal and commercial use (https://github.com/RosettaCommons/RFantibody).
- provenance note
- Created from the Bennett 2025 paper for the 2026-10-22 binder design pass.
- reported name
- fine-tuned RFdiffusion
- review
- artifact sha256: d7876bc73c47f2cb3fcb0fa1ef917c89c9b67750a22f6402bf515f978cd1564a; method: source-hash-verification; independent-cell-check; ai-assisted-source-review; method note: Re-downloaded from Europe PMC (HTTP 200, 169,840 bytes) and the SHA-256 recomputed and matched. Own flattening of the JATS XML. The main text's '95 designs per target' was confirmed verbatim and the string '96 designs' does not occur, so the conflict with Supplementary Methods Table 6 is real.; note: Reviewed in docs/reviews/use-cases/binder-2026-10-22.md. Automated AI review of the source transcription only; it is not human scientific review and nothing here was reproduced experimentally.; retrieval url: https://www.ebi.ac.uk/europepmc/webservices/rest/PMC12727541/fullTextXML; reviewed at: 2026-10-22T15:00:00Z; reviewer: claude; reviewer note: Claude review agent, independent of the extraction agent; no human review claimed
- source locator
- Results paragraph 1; Fig. 1; Code availability
- version
- As published in Nature 2025 (doi 10.1038/s41586-025-09721-5); released as RosettaCommons/RFantibody