Harmonised mNGS analysis workflow (Hu et al. 2026)
Host removal against GRCh38 plus plasmids, alignment of non-host reads to a curated 20,484-genome microbial database and lowest-common-ancestor assignment, with platform-specific normalised read-count thresholds and a historical negative-control background filter.
Results are recorded on linked configurations and versions of this method. Their settings and evaluations are kept separate below.
Results on linked records
This method has no results of its own in this release. 2 linked records have results. They are not assigned to the underlying method: each record keeps its own settings and evaluations, and its scores are on its own page.
- Host-depleted ONT GridION mNGS workflow (Hu et al. 2026) Configuration · configuration · 3 evaluations · 54 results
- MGISEQ-200 short-read mNGS workflow (Hu et al. 2026) Configuration · configuration · 3 evaluations · 54 results
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Sources and history
Release 2026-10-22-1b7cf027e4f6 · Record review: source checked
1 source record and release history
- Benchmarking next- versus third-generation sequencing in metagenomics: performance metrics and diagnostic efficacy · Original source · Microbiology Spectrum 14(7):e03993-25, published 2026-06-03; PMC13339908 full-text XML
Technical metadata and extraction receipts
Stable ID: dna-pathogen-20261011-hu2026-method-harmonised-mngs
- areas
- microbes-communities
- contexts
- clinical_research
- method types
- conventional_pipeline
- reported name
- harmonized analytic framework
- entity level
- method
- source locator
- Methods 'Bioinformatic processing and taxonomic assignment', 'Reference database curation' and 'Read normalization, reporting threshold, and contamination control'
- access
- Not stated; no code or database release is named in the article
- missing metadata
- version: reason: unreported; note: No software or database version is given for the workflow as a whole
Related records
- configuration of: Host-depleted ONT GridION mNGS workflow (Hu et al. 2026)
- configuration of: MGISEQ-200 short-read mNGS workflow (Hu et al. 2026)