rewirebio.iobenchmarks
Evaluation

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

Published somatic caller comparison; transcribed, not reproduced.

Research readiness

These checks assess whether the evidence supports a reproducible investigation. A source-checked score alone does not meet these requirements.

Release 2026-10-09-ba02f2f4a36e · Evidence verified: Not verified

Evidence incomplete

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Verified: Not verified

Evidence incomplete

Investigate discrepancies

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Verified: Not verified

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A pinned recipe describes the inputs, environment and resource requirements.

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Verified: Not verified

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Verified: Not verified

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Read reviewed discrepancy investigations

Evaluation results

1 evaluation · 6 results. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
0.733 f1-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), H12; Tools 'Varscan2'; column 'F1'
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
75 false-positive-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), E12; Tools 'Varscan2'; column 'FP'
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
4.98e-7 false-positive-rate
fraction · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), G12; Tools 'Varscan2'; column 'FPR'
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
0.905 precision
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), I12; Tools 'Varscan2'; column 'PPV'
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
0.616 recall
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), F12; Tools 'Varscan2'; column 'TPR'
Configuration: Varscan2 2.3.9 (Guille et al. 2025)Protocol: SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset: SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
715 true-positive-count
count · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Varscan2 on SEQC2 HCC1395 WES validation SNVs (Guille et al. 2025)

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Aggregation: Not reported

A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing; Guille et al. 2025, Supplementary Table S7 (validation dataset) · Supplementary Table S7 (tables7_bbae697.xls, sheet 'Results'), D12; Tools 'Varscan2'; column 'TP'

Source checking is not independent reproduction. Release 2026-10-09-ba02f2f4a36e.

Evaluation procedure

somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv

Configuration
Varscan2 2.3.9 (Guille et al. 2025)
Protocol
SEQC2 HCC1395 WES validation sample, somatic SNVs (Guille et al. 2025 Table S7)
Dataset
SEQC2 HCC1395/HCC1395BL WES, Fudan replicate (WES_FD_T_1), high-confidence regions
origin
Independent external evaluation
configuration
Primary source as retrieved 2026-10-09
protocol id
somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv
dataset version
Not reported
split
Held-out validation sample
population
1160 truth SNVs in high-confidence regions
inputs
Tumour-normal WES, 76x tumour (Table 2), bwa mem alignment
adaptation
Not reported
metric implementation
Not reported
aggregation
Pooled over the target regions of one pair
budget
Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split
Held-out validation sample
Adaptation
Not reported
Scoring implementation
Not reported

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

36 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-09-ba02f2f4a36e
Property and statementOriginal source and locationReview and provenance
attributes.comparison.adaptation
Not reported
Context-only references
A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Briefings in Bioinformatics 26(1):bbae697, published online 2025-01-18; PMC11790059 full-text XML
Retrieved: 2026-10-09T19:56:52Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 2c6fc6f329f7ebea34889de5f0cc8dff2d0d90b9a8dc3192ddac0949281c9827

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.adaptation
Not reported
Context-only references
Guille et al. 2025, Supplementary Table S7 (validation dataset)

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: tables7_bbae697.xls inside the Europe PMC supplementary files bundle for PMC11790059
Retrieved: 2026-10-09T20:08:20Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 735470fcb1ae7c3578ab3efefe12fde4b73bac279a1e7185950e5c2c0f1073f2

Hash scope: SHA-256 of tables7_bbae697.xls. Retrieved as one member of the Europe PMC supplementaryFiles zip. The zip is assembled per request (member timestamps equal the request time), so only the member file hash is pinned.

Archive member: tables7_bbae697.xls

Inspected artifact

attributes.comparison.aggregation
Pooled over the target regions of one pair
Context-only references
A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Briefings in Bioinformatics 26(1):bbae697, published online 2025-01-18; PMC11790059 full-text XML
Retrieved: 2026-10-09T19:56:52Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 2c6fc6f329f7ebea34889de5f0cc8dff2d0d90b9a8dc3192ddac0949281c9827

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.aggregation
Pooled over the target regions of one pair
Context-only references
Guille et al. 2025, Supplementary Table S7 (validation dataset)

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: tables7_bbae697.xls inside the Europe PMC supplementary files bundle for PMC11790059
Retrieved: 2026-10-09T20:08:20Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 735470fcb1ae7c3578ab3efefe12fde4b73bac279a1e7185950e5c2c0f1073f2

Hash scope: SHA-256 of tables7_bbae697.xls. Retrieved as one member of the Europe PMC supplementaryFiles zip. The zip is assembled per request (member timestamps equal the request time), so only the member file hash is pinned.

Archive member: tables7_bbae697.xls

Inspected artifact

attributes.comparison.budget
Not reported
Context-only references
A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Briefings in Bioinformatics 26(1):bbae697, published online 2025-01-18; PMC11790059 full-text XML
Retrieved: 2026-10-09T19:56:52Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 2c6fc6f329f7ebea34889de5f0cc8dff2d0d90b9a8dc3192ddac0949281c9827

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.budget
Not reported
Context-only references
Guille et al. 2025, Supplementary Table S7 (validation dataset)

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: tables7_bbae697.xls inside the Europe PMC supplementary files bundle for PMC11790059
Retrieved: 2026-10-09T20:08:20Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 735470fcb1ae7c3578ab3efefe12fde4b73bac279a1e7185950e5c2c0f1073f2

Hash scope: SHA-256 of tables7_bbae697.xls. Retrieved as one member of the Europe PMC supplementaryFiles zip. The zip is assembled per request (member timestamps equal the request time), so only the member file hash is pinned.

Archive member: tables7_bbae697.xls

Inspected artifact

attributes.comparison.dataset_version
Not reported
Context-only references
A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Briefings in Bioinformatics 26(1):bbae697, published online 2025-01-18; PMC11790059 full-text XML
Retrieved: 2026-10-09T19:56:52Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 2c6fc6f329f7ebea34889de5f0cc8dff2d0d90b9a8dc3192ddac0949281c9827

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.dataset_version
Not reported
Context-only references
Guille et al. 2025, Supplementary Table S7 (validation dataset)

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: tables7_bbae697.xls inside the Europe PMC supplementary files bundle for PMC11790059
Retrieved: 2026-10-09T20:08:20Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 735470fcb1ae7c3578ab3efefe12fde4b73bac279a1e7185950e5c2c0f1073f2

Hash scope: SHA-256 of tables7_bbae697.xls. Retrieved as one member of the Europe PMC supplementaryFiles zip. The zip is assembled per request (member timestamps equal the request time), so only the member file hash is pinned.

Archive member: tables7_bbae697.xls

Inspected artifact

attributes.comparison.inputs
Tumour-normal WES, 76x tumour (Table 2), bwa mem alignment
Context-only references
A benchmarking study of individual somatic variant callers and voting-based ensembles for whole-exome sequencing

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: Briefings in Bioinformatics 26(1):bbae697, published online 2025-01-18; PMC11790059 full-text XML
Retrieved: 2026-10-09T19:56:52Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 2c6fc6f329f7ebea34889de5f0cc8dff2d0d90b9a8dc3192ddac0949281c9827

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.inputs
Tumour-normal WES, 76x tumour (Table 2), bwa mem alignment
Context-only references
Guille et al. 2025, Supplementary Table S7 (validation dataset)

Original source ↗

Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: tables7_bbae697.xls inside the Europe PMC supplementary files bundle for PMC11790059
Retrieved: 2026-10-09T20:08:20Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 735470fcb1ae7c3578ab3efefe12fde4b73bac279a1e7185950e5c2c0f1073f2

Hash scope: SHA-256 of tables7_bbae697.xls. Retrieved as one member of the Europe PMC supplementaryFiles zip. The zip is assembled per request (member timestamps equal the request time), so only the member file hash is pinned.

Archive member: tables7_bbae697.xls

Inspected artifact

Sources and history

Release 2026-10-09-ba02f2f4a36e · Record review: source checked

2 source records and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: somatic-20261009-eval-guille2025-varscan2-seqc2-fd-wes-snv

areas
dna-genomes
contexts
clinical_research
origin
independent_paper
protocol
somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv
version
Primary source as retrieved 2026-10-09
comparison
protocol id: somatic-20261009-protocol-guille2025-seqc2-fd-wes-snv; dataset version: Not reported; split: Held-out validation sample; population: 1160 truth SNVs in high-confidence regions; inputs: Tumour-normal WES, 76x tumour (Table 2), bwa mem alignment; adaptation: Not reported; metric implementation: Not reported; aggregation: Pooled over the target regions of one pair; budget: Not reported
source locator
Supplementary Table S7 row 12 (Tools 'Varscan2', Type of variant 'SNV')
missing metadata
comparison.dataset version: reason: unreported; comparison.metric implementation: reason: unreported
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