rewirebio.iobenchmarks
Evaluation

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

Published DNA metagenomic classification comparison; transcribed, not reproduced.

Research readiness

These checks assess whether the evidence supports a reproducible investigation. A source-checked score alone does not meet these requirements.

Release 2026-10-09-8cc1db47c7f9 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

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Verified: Not verified

Evidence incomplete

Investigate discrepancies

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Missing or unresolved evidence

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Verified: Not verified

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Run locally

A pinned recipe describes the inputs, environment and resource requirements.

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Verified: Not verified

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Separate data and exposure records support an independent test.

Missing or unresolved evidence

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Verified: Not verified

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No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Read reviewed discrepancy investigations

Evaluation results

1 evaluation · 8 results. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
0.95 f-beta-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'F0.5'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
0.89 f1-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'F1'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
2 false-negative-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'False negatives'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
0 false-positive-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'False positives'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
20.3% composition-l1-distance
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'L1'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
1 precision
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'Precision'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
0.8 recall
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'Recall'
Configuration: mOTUs (Portik et al. 2022)Protocol: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
8 true-positive-count
count · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

mOTUs on ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42), column 'True positives'

Source checking is not independent reproduction. Release 2026-10-09-8cc1db47c7f9.

Evaluation procedure

dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species

Configuration
mOTUs (Portik et al. 2022)
Protocol
ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION): species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset
ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
origin
Independent external evaluation
configuration
Primary source as retrieved 2026-10-09
protocol id
dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species
dataset version
https://lomanlab.github.io/mockcommunity/r10.html (R10.3 release, February 2020)
split
Single sequencing run
population
10 scored species
inputs
ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
adaptation
None; reference databases as listed in Table 2
metric implementation
Authors' scoring of species read counts against the community composition
aggregation
Single dataset
budget
Not reported

Metadata review: source checked. Unreported conditions prevent automatic comparisons.

Reproduction

Split
Single sequencing run
Adaptation
None; reference databases as listed in Table 2
Scoring implementation
Authors' scoring of species read counts against the community composition

No execution recipe has been verified for this exact configuration and evaluation. A benchmark's general instructions may use different inputs, splits or model settings.

Reproducing this published result requires matching its model configuration, data, split and scorer. Source checking or a successful smoke test does not establish score reproduction.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

18 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-09-8cc1db47c7f9
Property and statementOriginal source and locationReview and provenance
attributes.comparison.adaptation
None; reference databases as listed in Table 2
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.adaptation

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.aggregation
Single dataset
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.aggregation

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.budget
Not reported
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

missing or unspecified

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.budget

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.dataset_version
https://lomanlab.github.io/mockcommunity/r10.html (R10.3 release, February 2020)
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.dataset_version

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.inputs
ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION)
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.inputs

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.metric_implementation
Authors' scoring of species read counts against the community composition
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.metric_implementation

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.population
10 scored species
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.population

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.protocol_id
dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.protocol_id

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.comparison.split
Single sequencing run
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.comparison.split

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.origin
independent_paper
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

independent paper

Audit details

Field: attributes.origin

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

Release 2026-10-09-8cc1db47c7f9 · Record review: source checked

1 source records and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261009-eval-portik2022-ont-r10-zymo-d6300-motus2-3-0-3

areas
microbes-communities
contexts
clinical_research
origin
independent_paper
protocol
dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species
version
Primary source as retrieved 2026-10-09
comparison
protocol id: dna-pathogen-20261009-protocol-portik2022-ont-r10-zymo-d6300-species; dataset version: https://lomanlab.github.io/mockcommunity/r10.html (R10.3 release, February 2020); split: Single sequencing run; population: 10 scored species; inputs: ONT R10.3 ZymoBIOMICS D6300 standard, length-filtered (GridION); adaptation: None; reference databases as listed in Table 2; metric implementation: Authors' scoring of species read counts against the community composition; aggregation: Single dataset; budget: Not reported
source locator
Table 4, dataset block 'ONT R10 Zymo D6300 (10 species, even)', row 'mOTUs' (table row 42)
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