rewirebio.iobenchmarks
Dataset

Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

Public mock community sequencing run as used in Portik et al. 2022.

Research readiness

These checks assess whether the evidence supports a reproducible investigation. A source-checked score alone does not meet these requirements.

Release 2026-10-09-8cc1db47c7f9 · Evidence verified: Not verified

Evidence incomplete

Replay metrics

Exact outcomes, predictions, identifiers and evaluator are connected.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • artifact hashes: verification is missing
  • join integrity: verification is missing
  • score semantics: verification is missing
  • metric replay: verification is missing

Verified: Not verified

Evidence incomplete

Investigate discrepancies

Replay evidence includes annotations and an assessment of dependence. Unknown independence permits descriptive analysis only.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • artifact hashes: verification is missing
  • join integrity: verification is missing
  • score semantics: verification is missing
  • metric replay: verification is missing
  • annotations: verification is missing
  • dependence: verification is missing

Verified: Not verified

Evidence incomplete

Run locally

A pinned recipe describes the inputs, environment and resource requirements.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • artifact hashes: verification is missing
  • join integrity: verification is missing
  • score semantics: verification is missing
  • recipe pinned: verification is missing
  • resource estimate: verification is missing

Verified: Not verified

Evidence incomplete

Validate independently

Separate data and exposure records support an independent test.

Missing or unresolved evidence

  • No verified artifact manifest is linked to this exact record.
  • artifact hashes: verification is missing
  • join integrity: verification is missing
  • score semantics: verification is missing
  • independent validation: verification is missing
  • overlap checked: verification is missing

Verified: Not verified

Readiness describes the evidence in this release. Availability on your computer is checked separately when an investigation runs. Existing data exposure can prevent independent validation even when files are available.

Artifacts and reproduction

No verified artifact manifest is connected to this record yet. The gaps above identify what is needed before analysis can begin.

Read reviewed discrepancy investigations

Evaluation results

7 evaluations · 56 results. Different protocols are not a single leaderboard.

Filter evaluations

Applied filters: All linked evaluations

Exact evaluated configurations and original reported results
Tested configurationProtocol and datasetFindingEvidence and details
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.11 f-beta-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'F0.5'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.17 f1-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'F1'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0 false-negative-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'False negatives'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
96 false-positive-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'False positives'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
80% composition-l1-distance
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'L1'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.09 precision
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'Precision'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
1 recall
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'Recall'
Configuration: Bracken (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
10 true-positive-count
count · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Bracken on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Bracken' (table row 66), column 'True positives'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.27 f-beta-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'F0.5'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.36 f1-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'F1'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
2 false-negative-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'False negatives'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
27 false-positive-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'False positives'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
81.7% composition-l1-distance
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'L1'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.23 precision
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'Precision'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.8 recall
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'Recall'
Configuration: Centrifuge-h22 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
8 true-positive-count
count · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Centrifuge-h22 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Centrifuge-h22' (table row 67), column 'True positives'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.17 f-beta-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'F0.5'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.24 f1-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'F1'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0 false-negative-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'False negatives'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
62 false-positive-count
count · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'False positives'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
59.7% composition-l1-distance
percent · lower

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'L1'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.14 precision
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'Precision'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
1 recall
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'Recall'
Configuration: Kraken2 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
10 true-positive-count
count · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

Kraken2 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'Kraken2' (table row 65), column 'True positives'
Configuration: MetaPhlAn3 (Portik et al. 2022)Protocol: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled: species detection at 0.001% of total reads (Portik et al. 2022 Table 4)
Dataset: Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
0.83 f-beta-score
fraction · higher

Uncertainty: Not reported by the source

Coverage: Not reported scored / Not reported eligible

Independent external evaluation · Source checked
Methods, coverage and source

MetaPhlAn3 on Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled

dna-pathogen-20261009-protocol-portik2022-illumina-zymo-d6300-species

Aggregation: Not reported

Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets · Table 4, dataset block 'Illumina Zymo D6300 (10 species, even)', row 'MetaPhlAn3' (table row 68), column 'F0.5'

Source checking is not independent reproduction. Release 2026-10-09-8cc1db47c7f9.

Dataset and evaluation context

A dataset supplies biological observations. The evaluation protocol defines how those observations are split, used and scored.

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

8 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-09-8cc1db47c7f9
Property and statementOriginal source and locationReview and provenance
attributes.accession
NCBI SRX8824472
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.accession

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.population
ZymoBIOMICS D6300: 10 species (8 bacteria at 12%, 2 yeasts at 2%). Subsampled to 20,000,000 of about 103 million 150 bp paired-end reads, 2.99 Gb.
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.population

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.scope_note
Species metrics are scored against 10 species.
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.scope_note

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.source_locator
Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.source_locator

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.split
Single sequencing run of one mock community; no split
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.split

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

attributes.version
As described in Portik et al. 2022 Table 1 (NCBI SRX8824472)
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: attributes.version

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

description
Public mock community sequencing run as used in Portik et al. 2022.
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: description

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

name
Illumina ZymoBIOMICS D6300 standard (NovaSeq 6000), subsampled
Context-only references
Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets

Original source ↗

Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4

Version: BMC Bioinformatics 23:541, published 2022-12-13; PMC9749362 full-text XML
Retrieved: 2026-10-09T19:45:04Z

not individually reviewed

No individual claim review recorded

Audit details

Field: name

Source artifact SHA-256: 42cf6834ec87e149752176a65247f3aab9873f7b215037a195175a8a3113c1fe

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

Release 2026-10-09-8cc1db47c7f9 · Record review: source checked

1 source records and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: dna-pathogen-20261009-data-portik2022-illumina-zymo-d6300

areas
microbes-communities
contexts
clinical_research
version
As described in Portik et al. 2022 Table 1 (NCBI SRX8824472)
accession
NCBI SRX8824472
population
ZymoBIOMICS D6300: 10 species (8 bacteria at 12%, 2 yeasts at 2%). Subsampled to 20,000,000 of about 103 million 150 bp paired-end reads, 2.99 Gb.
split
Single sequencing run of one mock community; no split
source locator
Table 1 row 'Illumina Zymo D6300'; Methods 'Mock community datasets' paragraph 4
scope note
Species metrics are scored against 10 species.
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