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Evidence claim

Relevance of uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3 to "Which computational design workflow produces binders that work when they are made and tested?"

Use case
Design a new binder to a chosen target
Assessed by
AlphaProteo yeast-display binding screen, hit at binding signal above 1.3 (IL-17A)
Relevance
Direct evidence
Endpoint
Share of tested AlphaProteo designs whose yeast-display binding signal exceeds 1.3, on IL-17A

The same measured endpoint as the 0.2-threshold screen but with a different threshold, kept separate because the threshold is part of the hit definition. It is the clearest single illustration in the evidence that a hit rate moves with the threshold rather than only with the method.

2 reviewed evaluations

Evidence

Source checking verifies the cited claim or transcription. It does not establish independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-10-22-1b7cf027e4f6
Property and statementOriginal source and locationReview and provenance
Citation locations
1 values
  • locator
    Section S1.2.1; Table 1, column 'IL-17A'
    source id
    uc-binder-20261022-source-alphaproteo
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.citation_locators

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison group
miniprotein-binder-hit-rates
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison_group

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Comparison title
Miniprotein binder hit rates by target
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.comparison_title

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Constraints
2 values
  • A different hit threshold from the other seven AlphaProteo targets, so this value is not comparable with them.
  • Read the value with its printed denominator of 63 designs.
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.constraints

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Endpoint
Share of tested AlphaProteo designs whose yeast-display binding signal exceeds 1.3, on IL-17A
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.endpoint

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Claimed field
links:assessed_by:uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.field

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Headline metric
hit-rate
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.headline_metric

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Limitations
5 values
  • Developer-reported.
  • The 1.3 threshold was set to account for background binding on this target and was calibrated by eye.
  • One target, one design system, 63 designs. No uncertainty is printed.
  • No conventional comparator.
  • Preprint, not peer reviewed.
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.limitations

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Pinned records
7 values
  • record id
    uc-binder-20261022-eval-alphaproteo-s2-rate-v1-il17a-main
    fields
    • status
    • links
    • attributes.origin
    • attributes.comparison
    sha256
    1c27d065f34a1c68c5b390e3ba6b176361bbe33a402ad6c03db26512fe7ffc82
  • record id
    uc-binder-20261022-eval-alphaproteo-table1-rate-il17a
    fields
    • status
    • links
    • attributes.origin
    • attributes.comparison
    sha256
    7d9fa9cae7428131ef6e9553159c960badad2e187f389152cc9bb84b69c2fe0e
  • record id
    uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3
    fields
    • status
    • name
    • description
    • facets
    • source_ids
    • links
    • attributes
    sha256
    021744e5333b3631285655a6822e1aa763ecbf09b4d475e30221d96b4186f7d4
  • record id
    uc-binder-20261022-result-alphaproteo-s2-hitrate-v1-il17a-main
    fields
    • status
    • attributes.metric
    • attributes.metric_qualifier
    • attributes.printed_value
    • attributes.numeric_value
    sha256
    ac429fe699efbc98af8d41b779524f88d084e31c2f121eb1b486287a09f9141c
  • record id
    uc-binder-20261022-result-alphaproteo-table1-hitrate-il17a
    fields
    • status
    • attributes.metric
    • attributes.metric_qualifier
    • attributes.printed_value
    • attributes.numeric_value
    sha256
    734ee5566c1d1827f0d5dfb8cc875e8f224b6bdbfaf347cf9714f473409e3b83
  • record id
    uc-binder-20261022-source-alphaproteo
    fields
    • status
    • attributes.artifact_sha256
    sha256
    4f4d69e2483d41e1016f2b3c6f078632280b3ca9ada6ac9f76e5ad7c16c914a0
  • record id
    use-case-de-novo-binder-design
    fields
    • status
    • name
    • description
    • facets
    • attributes.question
    • attributes.decision
    • attributes.inputs
    • attributes.output
    • attributes.exclusions
    • attributes.setting
    • attributes.clinical_scope
    • attributes.intended_users
    sha256
    b5a0012d1b0f02d6bd7466b7308123b26e676eda8ef54d706c865bb3f777e5b9
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.pins

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

Rationale
The same measured endpoint as the 0.2-threshold screen but with a different threshold, kept separate because the threshold is part of the hit definition. It is the clearest single illustration in the evidence that a hit rate moves with the threshold rather than only with the method.
Context-only references		De novo design of high-affinity protein binders with AlphaProteo (Zambaldi et al., arXiv 2024)

Original source ↗

uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'

Version: arXiv version 1, posted 2024-09-12; full-text PDF (49 pages)
Retrieved: 2026-10-22T11:53:00Z

not individually reviewed

No individual claim review recorded

Source has a recorded evidence concern. Consult its source page before using the claim.

Audit details

Field: attributes.rationale

Source artifact SHA-256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42

Hash scope: SHA-256 of one retrieval of the arXiv v1 PDF on 2026-10-22.

Inspected artifact

How to read the evidence

Sources and history

Release 2026-10-22-1b7cf027e4f6 · Record review: source checked

1 source record and release historyDownload this release (gzip)
Technical metadata and extraction receipts

Stable ID: use-case-mapping-binder-20261022-alphaproteo-yeast-display-signal-1-3

citation locators
locator: Section S1.2.1; Table 1, column 'IL-17A'; source id: uc-binder-20261022-source-alphaproteo
comparison group
miniprotein-binder-hit-rates
comparison title
Miniprotein binder hit rates by target
constraints
A different hit threshold from the other seven AlphaProteo targets, so this value is not comparable with them.; Read the value with its printed denominator of 63 designs.
endpoint
Share of tested AlphaProteo designs whose yeast-display binding signal exceeds 1.3, on IL-17A
field
links:assessed_by:uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3
headline metric
hit-rate
limitations
Developer-reported.; The 1.3 threshold was set to account for background binding on this target and was calibrated by eye.; One target, one design system, 63 designs. No uncertainty is printed.; No conventional comparator.; Preprint, not peer reviewed.
rationale
The same measured endpoint as the 0.2-threshold screen but with a different threshold, kept separate because the threshold is part of the hit definition. It is the clearest single illustration in the evidence that a hit rate moves with the threshold rather than only with the method.
reason
Add measured de novo binder design outcomes from the 2026-10-22 use-case evidence pass (#176).
relevance
direct
review
artifact sha256: 953b35644c0fa06ff090d7614c2e19525831f8fde50242e176d54474133c5a42; method: scope-review; independent-cell-check; ai-assisted-source-review; method note: Re-downloaded with curl (HTTP 200, 12,333,400 bytes) and the SHA-256 recomputed and matched. Own text layer made with pdftotext -layout (poppler 26.08.0), reproducing the extraction pass's 2,811 lines. Table 1, Table S2, Table S3 and Sections S1.2.1, S2, S4.1 and S4.2 read cell by cell and compared with every record. Column assignment re-derived arithmetically rather than trusted.; note: Reviewed in docs/reviews/use-cases/binder-2026-10-22.md. Automated AI review of the source transcription only; it is not human scientific review and nothing here was reproduced experimentally.; retrieval url: https://arxiv.org/pdf/2409.08022v1; reviewed at: 2026-10-22T15:00:00Z; reviewer: claude; reviewer note: Claude review agent, independent of the extraction agent; no human review claimed; scope: Endpoint fit, relevance grade, constraints, limitations, comparison grouping and the evaluations listed in reviewed_evaluations.
reviewed evaluations
uc-binder-20261022-eval-alphaproteo-s2-rate-v1-il17a-main; uc-binder-20261022-eval-alphaproteo-table1-rate-il17a
revision
1
source locator
uc-binder-20261022-source-alphaproteo: Section S1.2.1; Table 1, column 'IL-17A'
stratum label
AlphaProteo on IL-17A, yeast display at signal above 1.3
stratum order
2
value
uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3
pins
record id: uc-binder-20261022-eval-alphaproteo-s2-rate-v1-il17a-main; fields: status; links; attributes.origin; attributes.comparison; sha256: 1c27d065f34a1c68c5b390e3ba6b176361bbe33a402ad6c03db26512fe7ffc82; record id: uc-binder-20261022-eval-alphaproteo-table1-rate-il17a; fields: status; links; attributes.origin; attributes.comparison; sha256: 7d9fa9cae7428131ef6e9553159c960badad2e187f389152cc9bb84b69c2fe0e; record id: uc-binder-20261022-protocol-alphaproteo-yeast-display-signal-1-3; fields: status; name; description; facets; source_ids; links; attributes; sha256: 021744e5333b3631285655a6822e1aa763ecbf09b4d475e30221d96b4186f7d4; record id: uc-binder-20261022-result-alphaproteo-s2-hitrate-v1-il17a-main; fields: status; attributes.metric; attributes.metric_qualifier; attributes.printed_value; attributes.numeric_value; sha256: ac429fe699efbc98af8d41b779524f88d084e31c2f121eb1b486287a09f9141c; record id: uc-binder-20261022-result-alphaproteo-table1-hitrate-il17a; fields: status; attributes.metric; attributes.metric_qualifier; attributes.printed_value; attributes.numeric_value; sha256: 734ee5566c1d1827f0d5dfb8cc875e8f224b6bdbfaf347cf9714f473409e3b83; record id: uc-binder-20261022-source-alphaproteo; fields: status; attributes.artifact_sha256; sha256: 4f4d69e2483d41e1016f2b3c6f078632280b3ca9ada6ac9f76e5ad7c16c914a0; record id: use-case-de-novo-binder-design; fields: status; name; description; facets; attributes.question; attributes.decision; attributes.inputs; attributes.output; attributes.exclusions; attributes.setting; attributes.clinical_scope; attributes.intended_users; sha256: b5a0012d1b0f02d6bd7466b7308123b26e676eda8ef54d706c865bb3f777e5b9
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